Natalia V. Bykova, Ph.D.

Image Natalia V Bykova
Research Scientist

Cereal proteomics, mass spectrometry-based quantitative and functional posttranslational modification proteomics

Agriculture and Agri-Food Canada

Current research and/or projects

Research program focuses on functional modification-specific and quantitative proteomics; posttranslational coordination of signal transduction pathways induced in plants during biotic, abiotic stress response and seed development; quantitative mass spectrometry-driven technologies in plant proteomics to provide a deeper understanding of molecular mechanisms regulating the function and interaction of proteins.

Integrating quantitative proteomics, redox metabolomics and proteogenomics approaches to identify sources of pre-harvest sprouting resistance in Canadian wheat.

Mitigating emerging threats to wheat - Mexican durum rust threat. Discovery of nuclear-targeted effector proteins - quantitative nuclear proteomics of the host-pathogen interaction, integration of –omics and bioinformatics data, validation and functional analyses of candidate effectors including host targets / R-genes.

Research and/or project statements

Functional plant proteomics and mass spectrometry-based protein characterization, response to disease and stress

    Modification-specific proteomics - mass spectrometry-driven identification and characterisation of posttranslational modifications phosphorylation, glycosylation and cysteine oxidoreduction, and elucidation of modification-function relationships

    Determination of the role of posttranslational protein modifications in coordination of signal transduction pathways induced in plants during biotic, abiotic stress response and seed development

    Identification of signalling components and the molecular mechanisms of plant-pathogen interactions

Professional activities / interests

Member of Canadian and American Societies of Plant Biologists; International Plant Proteomics Organization (INPPO), Country Representative from Canada and Spokesperson in the Executive Council of INPPO (since 2011) that facilitates the development of new technologies in plant science, promotes plant proteomics, and intensifies international cooperation to integrate plant proteomics-related activities

Education and awards

Ph.D.  Biochemistry, Voronezh State University, Russia

M.Sc. Plant Biochemistry and Physiology, Voronezh State University, Russia

Postgraduate Studentship - G. Soros International Science Foundation, USA

International experience and/or work

Assistant Professor - Department of Biology, Memorial University of Newfoundland (MUN), 2008-2012

Research Associate - Mass spectrometry applications in biology, University of Manitoba, Physics, 2002-2004

Postdoctoral Position – Proteomics, Plant Research Department, Risø National Laboratory, Denmark, 2001-2002

Postdoctoral Fellowship - EU Biotechnology Framework IV, Umeå University, Department of Plant Physiology, Sweden, 1998-2001

Scholarship from the Swedish Institute (total 7 months) - Lund University, Department of Plant Physiology, Sweden, 1998

Key publications

For a complete list of publications, please visit: AAFC Online

Dumont S, Bykova NV, Khaou A, Besserour Y, Dorval M, Rivoal J (2018) Arabidopsis thaliana alcohol dehydrogenase is differently affected by several redox modifications. PLoS One, 13(9):e0204530. doi: 10.1371/journal.pone.0204530.

Igamberdiev AU, Bykova NV (2018) Role of organic acids in the integration of cellular redox metabolism and mediation of redox signalling in photosynthetic tissues of higher plants. Free Radical Biology and Medicine, 122:74-85. doi: 10.1016/j.freeradbiomed.2018.01.016.

Ma Z, Bykova NV, Igamberdiev AU (2017) Cell signaling mechanisms and metabolic regulation of germination and dormancy in barley seeds. The Crop Journal 5(6): 459-477.

Tailor S, Bykova NV, Igamberdiev AU, Samir C.  Debnath (2017) Structural pattern and genetic diversity in blueberry (Vaccinium) clones and cultivars using EST-PCR and microsatellite markers. Genetic Resources and Crop Evolution, pp 1–12. doi:10.1007/s10722-017-0497-1

Dumont S, Bykova NV, Pelletier G, Dorion S, Rivoal J (2016) Cytosolic triosephosphate isomerase from Arabidopsis thaliana is reversibly modified by glutathione on Cysteines 127 and 218. Frontiers in Plant Science 7: 1942. doi:  10.3389/fpls.2016.01942.

Fan T, Bykova NV, Rampitsch C, Xing T (2016) Identification and characterization of a serine protease from wheat leaves. European Journal of Plant Pathology 146: 293. doi:10.1007/s10658-016-0914-x.

Ma Z, Marsolais F, Bernards MA, Sumarah MW, Bykova NV, Igamberdiev AU. (2016) Glyoxylate cycle and metabolism of organic acids in the scutellum of barley seeds during germination. Plant Science, 248:37-44. doi: 10.1016/j.plantsci.2016.04.007.

Ma Z, Marsolais F, Bykova NV, Igamberdiev AU (2016) Nitric oxide and reactive oxygen species mediate metabolic changes in barley seed embryo during germination. Frontiers in Plant Science 7:138. doi: 10.3389/fpls.2016.00138.

Hu J, Rampitsch C, Bykova NV (2015) Advances in plant proteomics towards improvement of crop productivity and stress resistance. Invited review for the special issue on plant proteomics in Frontiers in Plant Science 6:209. doi: 10.3389/fpls.2015.00209.

Dong A, Bykova NV, Debnath SC (2015) EST-PCR, EST-SSR and ISSR markers to identify a set of wild cranberries and evaluating their relationships. Canadian Journal of Plant Science 95(6): 1155-1165. doi:10.4141/CJPS-2015-158.

Bykova NV,  Møller IM, , Gardeström P, Igamberdiev AU (2014) The function of glycine decarboxylase complex is optimized to maintain high photorespiratory flux via buffering of its reaction products. Mitochondrion 19B, 357-364. doi: 10.1016/j.mito.2014.01.001.

Ahmed AA, Balogun KA, Bykova NV, Cheema SK (2014) Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach. Nutrition & Metabolism (Lond) 11(1):6, pp11. doi: 10.1186/1743-7075-11-6.

Bykova NV, Rampitsch C (2013) Modulating protein function through reversible oxidation: a review of redox-mediated processes in plants. Invited review to the 2013 Annual Reviews Issue of Proteomics 13, 579–596, DOI 10.1002/pmic.201200270

Agrawal GK, Sarkar A, Righetti PG, Pedreschi R, Carpentier S, Wang T, Kohli A, Barkla BJ, Ndimba BK, Bykova NV, Rampitsch C, Zolla L, Rafudeen MS, Cramer R, Bindschedler LV, Tsakirpaloglou N, Ndimba RJ, Farrant JM, Renaut J, Job D, Kikuchi S, and Rakwal R (2013) A decade of plant proteomics and mass spectrometry: translation of technical advancements to food security and safety issues. Mass Spectrometry Reviews 32, 335–365, DOI 10.1002/mas, MAS-801(21365).

Rampitsch C, Bykova NV (2012) The beginnings of crop phosphoproteomics: exploring early warning systems of stress. Invited research topic review for Frontiers in Plant Science 3:144. doi: 10.3389/fpls.2012.00144

Debnath SC, Siow YL, Petkau J, An D, Bykova NV (2012) Molecular markers and anti-oxidant activity in berry crops genetic diversity analysis. Canadian Journal of Plant Science, Published on the web 10 July 2012, 10.4141/cjps2011-240

Rampitsch C, Bykova NV (2012) Proteomics and plant disease: Advances in combating a major threat to the global food supply. Proteomics 12: 673–690.

Bykova NV, Hoehn B, Rampitsch C, Hu J, Stebbing J-A, Knox R (2011) Thiol redox‐sensitive seed proteome in dormant and non‐dormant hybrid genotypes of wheat. Phytochemistry 72: 1162–1172.

Bykova NV, Hoehn B, Rampitsch C, Banks T, Stebbing J-A, Fan T, Knox R (2011) Redox-sensitive proteome and antioxidant strategies in wheat seed dormancy control. Proteomics 11: 865–882.

Igamberdiev AU, Bykova NV, Hill RD (2011) Structural and functional properties of class 1 plant hemoglobins. IUBMB Life 63: 146–152.

Rampitsch C, Subramaniam R, Djuric-Ciganovic S, Bykova NV (2010) The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation reveals phosphopeptides from proteins with a potential role in mycotoxin regulation. Proteomics 10: 124–140.

Igamberdiev A, Bykova NV, Shah JK, Hill RD (2010) Anoxic nitric oxide cycling in plants: participating reactions and possible mechanisms. Physiologia Plantarum 138 (2): 393–404.

Juszczuk IM, Bykova NV, Møller IM (2007). Protein phosphorylation in plant mitochondria. Physiologia Plantarum 129: 90-103.

Covey-Crump EM, Bykova NV, Affourtit C, Hoefnagel MHN, Gardetrom P, Atkin OK (2007) Temperature-dependent changes in respiration rates and redox poise of the ubiquinone pool in protoplasts and isolated mitochondria of potato leaves. Physiologia Plantarum 129: 175-184.

Bykova NV, Rampitsch C, Krokhin O, Standing KG, Ens W (2006) Determination and characterization of site-specific N-glycosylation using MALDI-Qq-TOF tandem mass spectrometry: case study with a plant protease. Analytical Chemistry 78(4): 1093-1103.

Bykova NV, Igamberdiev AU, Ens W, Hill RD (2006) Identification of an intermolecular disulfide bond in barley hemoglobin. Biochemical and Biophysical Research Communications 347 (1): 301-309.

Rampitsch C, Bykova NV, McCallum B, Beimcik E, Ens W (2006) Analysis of the wheat and Puccinia triticina (leaf rust) proteomes during a susceptible host-pathogen interaction. Proteomics 6(6): 1897-1907.

Rampitsch C, Bykova NV, Mauthe W, Yakandawala N, Jordan M (2006) Phosphoproteomic profiling of wheat callus labelled in vivo. Plant Science 171 (4): 488-496.

Igamberdiev A, Bykova NV, Hill R. (2006) Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate reductase-mediated ascorbate reduction of methemoglobin. Planta 223(5): 1033-1040.


Book Chapters:

Igamberdiev AU, Bykova NV (2019) Metabolism and Interplay of Reactive Oxygen and Nitrogen Species in Plant Mitochondria. In: Nitric Oxide and Hydrogen Peroxide Signaling in Higher Plants. D.K. Gupta, J. M. Palma, F. J. Corpas (Editors), Springer International Publishing AG Switzerland.; pp 91-106.

Bykova NV, Igamberdiev AU (2016) Redox state in plant mitochondria and its role in stress tolerance. In: Redox State as a Central Regulator of Plant-Cell Stress Responses. D.K. Gupta, J. M. Palma, F. J. Corpas (Editors), Springer International Publishing AG Switzerland. DOI 10.1007/978-3-319-44081-1_5; pp 93-115.

Igamberdiev AU, Bykova NV (2015) Non-coupled pathways of plant mitochondrial electron transport and the maintenance of high photorespiratory flux. In: Alternative Respiratory Pathways in Higher Plants. K.J. Gupta, L.A.J. Mur, B. Neelwarne (Editors). John Wiley and Sons, Oxford. ISBN 978-1-118-79046-5. DOI: 10.1002/9781118789971.ch2

Bykova NV, Hu J, Ma Z, Igamberdiev AU (2015) The role of reactive oxygen and nitrogen species in bioenergetics, metabolism and signaling during seed germination. In: Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants. Gupta KJ, Igamberdiev AU (Editors). Springer, Berlin. ISBN 978-3-319-10078-4 (hardcover), 978-3-319-10079-1 (ebook); pp 177-195.

Rampitsch C, Bykova NV (2009) Methods for Functional Proteomic Analyses, In: Plant Genomics: Methods and Protocols. Methods in Molecular Biology. Vol. 513. DJ Somers, Langridge P, Gustafson JP, eds, Humana Press; pp 93-110.

Bykova NV, Møller IM (2006) Proteomics of plant mitochondria, In: Plant Proteomics. Annual Plant Reviews. Vol. 28, C Finnie, ed., Blackwell Publishing; pp 211-243.