Mélissa Duplessis, Ph.D., Agronomist

Image Mélissa Duplessis
Research scientist

Dairy Production: Integration of cow nutrition, metabolism and health as well as environmental impacts of diets

Current research and/or projects

  • Ensuring ecosystem productivity and resiliency by assessing the impact of trace-metal contamination from manure on the potential for cyanobacterial and harmful algal blooms in fresh water bodies - Trials in commercial herds (Living lab) and in experimental herd;
  • Evaluate apparent absorption of trace metals and phosphorus between Holstein and Ayrshire cows;
  • Describe the actual practice in commercial dairy herds regarding trace-metal feeding;
  • Impact of vitamin B supplementation on energy and protein metabolism during the peripartum period of dairy cows;
  • Impact of cow nutrition on vitamin B12 concentration in milk.

Research and/or project statements

  • Trace metals and their impact on the ecosystem;
  • Better understand interactions between systems by integration of knowledge;
  • Vitamin B, minerals, energy and protein metabolism during the transition period of dairy cows;
  • Link between B-vitamin status and indicators of energy at the beginning of the lactation;
  • Impact of nutrition and metabolism on vitamin B12 concentration in milk of cow.

Publications

39-Duplessis, M., Terry, S. A., Pontes, L. dS. et Leytem, A. B. 2023. Editorial : Mitigating the impact of animal production on the environment: the ecosystem integration. Frontiers in Animal Science. 4:1289902.

38-Duplessis, M., Chorfi, Y. et Girard, C. L. 2023. Longitudinal data to assess relationships among plasma folate, vitamin B12, non-esterified fatty acid, and β-hydroxybutyrate concentrations of Holstein cows during the transition period. Metabolites. 13: 547.

37-Duplessis, M. et Royer, I. 2023. Mini-Review: The importance of an integrated approach to assess trace mineral feeding practices in dairy cows. Frontiers in Animal Science. 4:1155361.

36-Mion, B., Ogilvie, L., Van Winters, B., Spricigo, J. F., Anan, S., Duplessis, M., McBride, B. W., LeBlanc, S. J., Steele, M. A. et Ribeiro, E. S. 2023. Effects of replacing inorganic salts of trace minerals with organic trace minerals in the pre- and postpartum diets on mineral status, antioxidant biomarkers, and health of dairy cows. Journal of Animal Science. 101: 1-16. DOI 10.1093/jas/skad041.

35-Girard, C. L. et Duplessis, M. 2023. Review – State of knowledge on the importance of folates and cobalamin for dairy cow metabolism. Animal. 17:100834.

34-Duplessis, M., Wright, T. C. et Bejaei, M. 2023. A survey of Canadian dairy nutritionists to assess current trace element formulation practices. Journal of Dairy Science. 106:4030-4041. 

33-Duplessis, M., Lapierre, H. et Girard, C. L. 2022. Biotin, folic acid, and vitamin B12 supplementation given in early lactation to Holstein dairy cows: Their effects on whole-body propionate, glucose, and protein metabolism. Animal Feed Science and Technology. 292 :15441.

32-Duplessis, M., Martineau, R., Fadul-Pacheco, L. et Pellerin, D. 2022. Comparison of cow milk components between daily actual and AM-PM composite samples from two consecutive milkings by Dairy Herd Improvement. Applied Animal Science. 38: 350-359.  

31-Duplessis, M., Gervais, R., Lapierre, H. et Girard, C. L. 2022. Combined biotin, folic acid, and vitamin B12 supplementation given during the transition period to dairy cows: Part II. The impact on energy balance and fatty acid composition of colostrum and milk. Journal of Dairy Science. 105: 7097-7110.

30-Duplessis, M., Lapierre, H., Sauerwein, H. et Girard, C. L. 2022. Combined biotin, folic acid, and vitamin B12 supplementation given during the transition period to dairy cows: Part I. The impact on lactation performance, energy and protein metabolism, and hormones. Journal of Dairy Science. 105: 7079-7096.

29-Girard, C. L. et Duplessis, M. 2022. Invited review: The importance of B vitamins in precision nutrition of dairy cows: The case of folates and vitamin B12. Canadian Journal of Animal Science. 102: 201-210.

28-Duplessis, M., Fadul-Pacheco, L., Santschi, D. E. et Pellerin, D. 2021. Toward precision feeding regarding minerals : What is the current practice in commercial dairy herds in Québec, Canada? Animals. 11: 1320. DOI 10.3390/ani11051320.

27-Duplessis, M., Vasseur, E., Ferland, J., Pajor, E. A., DeVries, T. J. et Pellerin, D. 2021. Short communication : Performance perception of Canadian dairy producers when transitioning to an automatic milking system. JDS Communications Accepté

26-Duplessis, M., Fréchette, A., Poisson, W., Blais, L. et Ronholm, J. 2021. Refining knowledge on factors affecting vitamin B12 concentration in bovine milk. Animals. 11: 532. DOI 10.3390/ani11020532.

25-Duplessis, M. et Girard, C. L. 2021. Response to a glucose tolerance test in early lactation Holstein cows receiving a supplementation of biotin, folic acid, and vitamin B12. J. Dairy Sci. 104:1111-1122. DOI 10.3168/jds.2020-19333.

24-Duplessis, M., Pellerin, D., Girard, C. L., Santschi, D. E. et Soyeurt, H. 2020. Short Communication : Potential prediction of vitamin B12 concentration based on mid-infrared spectral data using Holstein dairy herd improvement milk samples. J. Dairy Sci. 103: 7540-7546. DOI 10.3168/jds.2019-17758.

23-McFadden, J. W., Girard, C. L., Tao, S., Zheng, Z., Bernard, J. K., Duplessis, M. et White, H. M. Symposium review: One-carbon metabolism and methyl donor nutrition in the dairy cow. J. Dairy Sci. 103: 5668-5683. DOI 10.3168/jds.2019-17319.

22-Franco-Lopez, J., Duplessis, M., Bui, A., Reymond, C., Poisson, W., Blais, L., Chong, J., Gervais, R., Rico, D. E., Cue, R. I., Girard, C. L. et Ronholm, J. 2020. Correlations between the composition of the bovine microbiota and vitamin B12 abundance. mSystems. 5:e00107-20. DOI 10.1128/mSystems.00107-20.  

21-Duplessis, M., Blais, L., Poisson, W. et Girard, C. L. 2020. Technical notes: Extrapolation of hepatic glycogen concentration of the whole organ by performing a liver biopsy. J. Dairy Sci. 103: 4858-4862. DOI 10.3168/jds.2019-17905.

20-Duplessis, M., Ritz, K. E., Socha, M. T. et Girard, C. L. 2020. Cross-sectional study of the impact of diet composition on plasma folate and vitamin B12 concentrations of Holstein cows in the United States and Canada. J. Dairy Sci. 103: 2883-2895. DOI 10.3168/jds.2019-17657.

19-Duplessis, M. et Girard, C. L. 2019. Effect of maternal biotin, folic acid, and vitamin B12 supplementation before parturition on colostral and Holstein calf plasma concentrations in those vitamins. Anim. Feed Sci. Technol. 256: 114241.

18-Girard, C. L., Vanacker, N., Beaudet, V., Duplessis, M. et Lacasse, P. 2019. Glucose and insulin responses to an intravenous glucose tolerance test administered to fed-restricted dairy cows receiving folic acid and vitamin B12 supplements. J. Dairy Sci. 102: 6226-6234.

17-Duplessis, M., Pellerin, D., Robichaud, R., Fadul-Pacheco, L. et Girard, C. L. 2019. Impact of diet management and composition on vitamin B12 concentration in milk of Holstein cows. Animal. 13: 2101-2109.

16-Duplessis, M., Lacroix, R., Fadul-Pacheco, L., Lefebvre, D. M. et Pellerin, D. 2019. Assessment of the Canadian model predicting daily milk yield and milk fat percentage using single-milking dairy herd improvement samples. Can. J. Anim. Sci. 99 : 521-531.

15-Duplessis, M., Santschi, D. E., Plante, S., Bergeron, C., Lefebvre, D. M., Durocher, J., Cue, R. I. 2019. Milk β-hydroxybutyrate concentration measured by Fourier-transform infrared and flow-injection analyses from samples taken at different times relative to milking. J. Dairy Res. 86: 208-210.

14-Duplessis, M., Cue, R. I, Santschi, D. E., Lefebvre, D. M. et Girard, C. L. 2018. Short communication : Relationships among plasma and milk vitamin B12, plasma free fatty acids, and blood β-hydroxybutyrate concentrations in early lactation dairy cows. J. Dairy Sci. 101: 8559-8565.

13-Duplessis, M., Lapierre, H., Ouattara, B., Bissonnette, N., Pellerin, D., Laforest, J.-P. et Girard, C. L. 2017. Whole-body propionate and glucose metabolism of multiparous dairy cows receiving folic acid and vitamin B12 supplements. J. Dairy Sci. 100: 8578-8589.

12-Fadul-Pacheco, L., Pellerin, D., Chouinard, P. Y., Wattiaux, M. A., Duplessis, M. et Charbonneau, É. 2017. Nitrogen efficiency of commercial dairy herds : Impact on production performance and farm profitability. J. Dairy Sci. 100: 6592-6601.

11-Duplessis, M., Lapierre, H., Pellerin, D., Laforest, J.-P. et Girard, C. L. 2017. Effects of intramuscular injections of folic acid, vitamin B12, or both, on lactational performance and energy status of multiparous dairy cows. J. Dairy Sci. 100: 4051-4064.

10-Santschi, D. E., Lacroix, R., Durocher, J., Duplessis, M., Moore, R. K. et Lefebvre, D. M. 2016. Prevalence of elevated milk β-hydroxybutyrate concentrations in Holstein cows measured by FTIR analysis in Dairy Herd Improvement milk samples and association with milk yield and components. J. Dairy Sci. 99: 9263-9270.

9-Ouattara, B., Bissonnette, N., Duplessis, M. et Girard, C. L. 2016. Supplements of vitamins B9 and B12 affect hepatic and mammary gland gene expression profiles in lactating dairy cows. BMC Genomics. 17: 640.

8-Duplessis, M., Pellerin, D., Cue, R. I. et Girard, C. L. 2016. Short communication: Factors affecting vitamin B12 concentration in milk of commercial dairy herds: An exploratory study. J. Dairy Sci. 99: 4886-4892.

7-Mann, S., Leal Yepes, F. A., Duplessis, M., Wakshlag, J. J., Overton, T. R., Cummings, B. P. et Nydam, D. V. 2016. Dry period plane of energy: effects on glucose tolerance in transition dairy cows. J. Dairy Sci. 99: 701-717.

6-Duplessis, M., Mann, S., Nydam, D. V., Girard, C. L., Pellerin, D. et Overton, T. R. 2015. Short communication: Folates and vitamin B12 in colostrum and milk from dairy cows fed different energy levels during the dry period. J. Dairy Sci. 98: 5454-5459.

5-Duplessis, M., Cue, R. I., Santschi, D. E., Lefebvre, D. M. et Lacroix, R. 2015. Weight, height and relative-reliability indicators as a management tool for reducing age at first breeding and calving of dairy heifers. J. Dairy Sci. 98:2063-2073.

4-Duplessis, M., Girard, C. L., Santschi, D. E. et Pellerin, D. 2014. An economic model evaluating the supplementation of folic acid and vitamin B12 given around parturition and in early lactation on dairy farms in Québec, Canada. Can. J. Anim. Sci. 94:737-747.

3-Duplessis, M., Girard, C. L., Santschi, D. E., Lefebvre D. M. et Pellerin, D. 2014. Milk production and composition, and body measurements of dairy cows receiving intramuscular injections of folic acid and vitamin B12 in commercial dairy herds. Livest. Sci. 167: 186-194.

2-Duplessis, M., Girard, C. L., Santschi, D. E., Laforest, J.-P., Durocher, J. et Pellerin, D. 2014. Effects of folic acid and vitamin B12 supplementation on culling rate, diseases, and reproduction in commercial dairy herds. J. Dairy Sci. 97: 2346-2354.

1-Ouattara, B., Duplessis, M. et Girard, C. L. 2013. Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity. BMC Biochem. 14: 25-35.

Professional activities / interests

  • Vitamins and Minerals
  • Precision nutrition
  • Metabolism
  • Smart dairy farming

Education and awards

Mention on the Dean's list of Université Laval for my Ph. D. thesis and defense (Tableau d'honneur de la Faculté des études supérieures et postdoctorales de l'Université Laval), 2014

Ph. D. in Animal Sciences, Université Laval, 2014

B. Sc. in Agronomy oriented in Animal Sciences, Université Laval, 2009

International experience and/or work

Internship during my Ph. D. at Cornell University, NY, USA with Dr. Tom Overton from Department of Animal Science.

Post-doctoral internship at Lactanet, Canadian Network for Dairy Excellence, Sainte-Anne-de-Bellevue, Canada. 

Collaboration with Dr. Hélène Soyeurt from Université de Liège, Belgium for a project aiming to predict vitamin B12 concentration in milk using mid-infrared spectral data.

Key publications

  1. Duplessis, M., Blais, L., Poisson, W., Girard, C.L. (2020). Technical note: Extrapolation of hepatic glycogen concentration of the whole organ by performing a liver biopsy. Journal of Dairy Science (JDS), [online] 103(5), 4858-4862. http://dx.doi.org/10.3168/jds.2019-17905

    2020 - View publication details

  2. Girard, C.L., Vanacker, N., Beaudet, V., Duplessis, M., Lacasse, P. (2019). Glucose and insulin responses to an intravenous glucose tolerance test administered to feed-restricted dairy cows receiving folic acid and vitamin B12 supplements. Journal of Dairy Science (JDS), [online] 102(7), 6226-6234. http://dx.doi.org/10.3168/jds.2019-16298

    2019 - View publication details

  3. Girard, C. L., Vanacker, N., M. Duplessis and P. Lacasse. 2017. Effects of supplementary folic acid and vitamin B12 on glucose and insulin responses of fed-restricted dairy cows to an intravenous glucose tolerance test. Journal of Dairy Science Vol. 100, Suppl. 2: 299

    2018 - View publication details

  4. Duplessis, M., Mann, S., Nydam, D.V., Girard, C.L., Pellerin, D., Overton, T.R. (2015). Short communication: Folates and vitamin B12 in colostrum and milk from dairy cows fed different energy levels during the dry period. Journal of Dairy Science (JDS), [online] 98(8), 5454-5459. http://dx.doi.org/10.3168/jds.2015-9507

    2015 - View publication details

  5. Duplessis, M., Girard, C.L., Santschi, D.E., Pellerin, D. (2014). An economic model evaluating the supplementation of folic acid and vitamin B12 given around parturition and in early lactation on dairy farms in Québec, Canada. Canadian Journal of Animal Science, [online] 94(4), 737-747. http://dx.doi.org/10.4141/CJAS-2014-026

    2014 - View publication details

  6. Duplessis, M., Girard, C.L., Santschi, D.E., Laforest, J.P., Durocher, J., Pellerin, D. (2014). Effects of folic acid and vitamin B12 supplementation on culling rate, diseases, and reproduction in commercial dairy herds. Journal of Dairy Science (JDS), [online] 97(4), 2346-2354. http://dx.doi.org/10.3168/jds.2013-7369

    2014 - View publication details

  7. Duplessis, M., Girard, C.L., Santschi, D.E., Lefebvre, D.M., Pellerin, D. (2014). Milk production and composition, and body measurements of dairy cows receiving intramuscular injections of folic acid and vitamin B-12 in commercial dairy herds. Livestock Science, [online] 167(1), 186-194. http://dx.doi.org/10.1016/j.livsci.2014.06.022

    2014 - View publication details

  8. Ouattara, B., Duplessis, M., Girard, C.L. (2013). Optimization and validation of a reversed-phase high performance liquid chromatography method for the measurement of bovine liver methylmalonyl-coenzyme a mutase activity. BMC Biochemistry, [online] 14(1), http://dx.doi.org/10.1186/1471-2091-14-25

    2013 - View publication details

Research facility

2000 College Street
Sherbrooke, QC J1M 0C8
Canada

Affiliations

Member of Ordre des agronomes du Québec

Member of the American Dairy Science Association

Associate professor at Département de biomédecine vétérinaire of Faculté de médecine vétérinaire of Université de Montréal

Associate professor at Départmement des sciences animales of Université Laval

Member of Op+Lait

Member of GRESABO

Language

English
French