Meenu K. Sharma, Ph.D.

Image Sarah London
Research Scientist

Conducts basic and applied mycobacteriology research focusing on Tuberculosis at the National Reference Centre for Mycobacteriology (TB lab)

Public Health Agency of Canada

Current research and/or projects

Dr. Sharma’s laboratory has established both national and international collaborations with her research in tuberculosis using animal models, and in diagnostic and applied research using the novel genotyping methodologies and databases.

Dr. Sharma’s laboratory plays an instrumental role in projects utilizing molecular methods, bacterial genomics, human genetic markers of tuberculosis, new technology platforms for testing, new algorithms, utilization of different genotyping methods for tracking tuberculosis outbreaks and TB control.

Research and/or project statements

  • Whole-genome Sequence-based Genotyping Approaches for Tuberculosis in Northern Canadian Communities
  • Lab work helps address the urgent need of a laboratory based TB genotyping surveillance in collaboration and participation from laboratories and public health units
  • Research and Development on new omics approaches to tackling drug resistant tuberculosis
  • Collaborating on whole genome sequencing-based genotyping for molecular epidemiology of Mycobacterium bovis infection in animals and humans in Canada
  • Characterized the strain dependent cytokine profile of Mycobacterium tuberculosis with focus on the Type 1 strain identification in Manitoba and defined the relationship between disease phenotype and the different mycobacterial strains

Professional activities / interests

Supervises co-op students, FSWEP, graduate students, medical microbiology trainees and post-doctoral fellows.

Delivered presentations on TB in India and cross-Canada

Education and awards

Education

Ph.D. Zoology, Panjab University, 1999

M.Sc. (Hons.) Zoology, Panjab University, 1994

B.Sc. (Hons.) Zoology, Panjab University, 1992

Awards

Appreciation award for contribution to Toronto TB outbreak in homeless, 2005

Public Service Award, Health Canada, 2002

Fellow, Natural Sciences Engineering and Research Council, Ottawa, 2001

International experience and/or work

Coauthor of international standards and technical reports

Experience in laboratory capacity building and training internationally

On International committees and working groups

Editor and/or reviewer for national and international scientific journals

Key publications

Book Chapters, Reports

B Forbes, M Miller, N Banaei, B Brown-Elliot, S Das, M Salfinger, M K Sharma, A Somoskovi, J Tans-Kersten, F C Tenoer, D Warshauer and A M Zelazny.

M48 - Laboratory detection and identification of mycobacteria. Clinical and laboratory Standards Institute. 2nd edition, September 2018.

Technical report on critical concentration for drug susceptibility testing of medicines used in the treatment of drug resistant tuberculosis. 2018. Geneva: World Health Organization; 2018 (WHO/CDS/TB/2018.5). Licence CC BY-NC-SA 3.0 IGO.

Major data contributors (K Andries, A Aubry, I Bastian, E Böttger, E Cambau, D Cirillo, P Claxton, E Desmond, J de Steenwinkel, K Dheda, A Diacon, D Dolinger, A Engström, M Farhat, L Fattorini, S Heysell, D Hillemann, H Hoffmann, E Houpt, P Hsueh, Y Hu, N Ismail, J Jani, K Kaniga, P Keller, I Laurenson, J Limberis, G Lin, Y Liu, A López-Gavín, H Mauch, S Omar, M Palaci, S Peacock,T Prammananan, J Posey, L Rigouts, J Robledo, C Rodrigues, T Schön, M Sharma, T Shinnick, F Sirgel, S Somasundaram, T Sterling, J van Ingen, D van Soolingen, A Venter, N Veziris, C Villellas, R Warren, J Werngren, C Yang, W W Yew and D Zimenkov).

S. Christianson, F. Jamieson, J. Wolfe, and M. Sharma

Appendix D: TUBERCULOSIS AND MYCOBACTERIOLOGY LABORATORY STANDARDS: SERVICES AND POLICIES.  In:  Canadian Tuberculosis Standards, 7th ed. 2014 Public Health Agency of Canada and the Canadian Lung Association/Canadian Thoracic Society.

 

Research Papers

Wuzinski, M., Bak, A.K., Petkau, A., (...), Soualhine, H., Sharma, M.K. A multilocus sequence typing scheme for Mycobacterium abscessus complex (MAB-multilocus sequence typing) using whole-genome sequencing data. 2019, International Journal of Mycobacteriology. 8(3), pp. 273-280. 

http://doi.org/10.4103/ijmy.ijmy_106_19

Chakravarti, A., Soualhine, H., Sharma, M., (...), Poirier, L., Lavallée, C.  Indigenous Transmission of Mycobacterium africanum in Canada: A Case Series and Cluster Analysis. 2019, Open Forum Infectious Diseases. 6(4).

http://doi.org/10.1093/ofid/ofz088

 Patel, S., Paulsen, C., Heffernan, C., Saunders, D., Sharma, M., King, M.,Long, R. (2017). Tuberculosis transmission in the Indigenous peoples of the Canadian prairies. PLoS ONE, 12(11), e0188189.

http://doi.org/10.1371/journal.pone.0188189   

Tyler AD, Randell E, Baikie M, Antonation K, Janella D, Christianson S, G Tyrrell, M Graham, G Van Domselaar, M K Sharma (2017) Application of whole genome sequence analysis to the study of M tuberculosis in Nunavut, Canada. PLoS ONE 12(10): e0185656.

http://doi.org/10.1371/10.1371/journal.pone.0185656  

S. Kiazyk, L. Larcombe, C. Lopez, S. Matyas, C. Mesa, P. Orr, J. Juno, J. Waruk, M. Sharma, T. B. Ball (2016). IFN-γ promoter polymorphisms do not affect QuantiFERON® TB Gold In-Tube test results in a Canadian population. The International Journal of Tuberculosis and Lung Disease 20(12):1647-1652.

http://doi.org/10.5588/ijtld.16.0223

Christianson S, Grierson W, Kein D, Tyler AD, Wolfe J, Sharma MK (2016). Time-to-Detection of Inducible Macrolide Resistance in Mycobacterium abscessus Subspecies and Its Association with the Erm(41) Sequevar. PLoS ONE 11(8): e0158723.

http://doi.org/10.1371/journal.pone.0158723

Tyler AD, Christianson S, Knox NC, Mabon P, Wolfe J, Van Domselaar G, M. Graham, M K Sharma (2016). Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis. PLoS ONE 11(2): e0148676. 

http://doi.org/10.1371/journal.pone.0148676

 Braun K, Wolfe J, Kiazyk S, Kaushal Sharma M. (2015). Evaluation of host genetics on outcome of tuberculosis infection due to differences in killer immunoglobulin-like receptor gene frequencies and haplotypes. BMC Genet. 2015 Jun 16;16:63.

http://doi.org/10.1371/journal.pone.0067842

 J. L. de Beer, C. Ko¨ dmo¨ n, J. van Ingen, P. Supply, D. van Soolingen, (2014). The Global Network for the Molecular Surveillance of Tuberculosis 2010 (includes M Sharma). Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex. INT J TUBERC LUNG DIS 18(5):594–600

http://doi.org/10.5588/ijtld.13.0531

 S. Christianson, D Voth, M. Sharma, J Wolfe. (2014). Re-evaluation of the critical concentration for ethambutol antimicrobial sensitivity testing on the MGIT 960. PLOS ONE. 2014 9(9):e108911.

http://doi.org/10.1371/journal.pone.0108911  

Cowan LS, Hooks DP, Christianson S, Sharma MK, Alexander DC, Guthrie JL, Jamieson FB, Supply P, Allix-Béguec C, Cruz L, Desmond E, Kramer R, Lugo S, Rudrik J. (2012)

Evaluation of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping as performed in laboratories in Canada, France, and the United States. J Clin Microbiol. 50(5):1830-1; author reply 1832.

http://doi.org/10.1128/JCM.00168-12

MK Sharma, Louise Thibert, Pamela Chedore, Cary Shandro, Frances Jamieson, Gregory Tyrrell, Sara Christianson, Hafid Soualhine, Joyce Wolfe. (2011)

A Canadian Multicentre Laboratory Study for Standardized Second-line Antimicrobial Susceptibility Testing of Mycobacterium  tuberculosis. Journal of Clinical Microbiology; 49: 4112-4116

http://doi.org/10.1128/JCM.05195-11

Petrelli D., MK Sharma, J. Wolfe, A. Al-Azem, E. Hershfield and A. Kabani (2004).

Strain‑related virulence of the dominant Mycobacterium tuberculosis strain in the Canadian province of Manitoba. Tuberculosis, 84(5):317‑26.

http://doi.org/10.1016/j.tube.2004.01.001         

Sharma, MK, A. Al-Azem, J. Wolfe, E. Hershfield, and A. Kabani (2003).

Identification of a predominant isolate of Mycobacterium tuberculosis using molecular and clinical epidemiology tools and in vitro cytokine responses. BMC-Infectious Diseases, 3: 3.

http://doi.org/10.1186/1471-2334-3-3

 

Affiliations

Adjunct Professor, Department of Medical Microbiology, Faculty of Graduate studies, University of Manitoba  

Language

English

Other languages

Hindi, punjabi