Marie-France Palin, Ph.D.

Current research and/or projects

I am studying interactions between genes and nutrients (e.g. polyunsaturated fatty acids, amino acids) in growing pigs with the aim of developing new nutrigenomic tools to improve carcass and meat quality and the mammary gland development.

I am also working on genomics and nutritional strategies to increase pork carnosine content. Carnosine is a naturally occurring dipeptide with recognized health attributes. Increasing muscle carnosine content in pigs would be associated with better meat quality and improved meat attributes.

Research and/or project statements

 Active research projects:

  • Enhancement of carnosine content and its nutritional advantages in pork.

  • New technologies to identify pigs with higher muscle carnosine, a new value-added attribute, and advantages of carnosine-enhanced pork in improving meat quality traits and nutritional value of the meat. 

  • Role of prolactin in the regulation of mammary development in late-gestating sows.

  • New feeding and management strategies for replacement gilts that will maximize future milk yield.

Professional activities / interests

  • Animal genomics and nutrigenomics.
  • Identification of marker genes associated with meat quality and mammary development.
  • Role of adipokines in mammary development and function.
  • In vitro cell and tissue culture models (adipose and muscle cells, liver tissue) to characterize different cellular mechanisms (e.g. lipogenesis, oxidative stress, cell growth and differentiation).

Education and awards

 

  • Ph.D. in Biochemistry, Sherbrooke University

  • B.Sc. in Microbiology, Sherbrooke University

  • Received the Young Scientist Award from the Canadian Society of Animal Science.

Key publications

  1. Salgado Romero, H.H., Palin, M.F., Lapierre, H., Remus, A., Létourneau-Montminy, M-P. and Pomar, C. (2021) Low insulin sensitivity is associated with increased body fat and changes in gene expression of lipogenic enzymes in the adipose tissue of finishing pigs. Journal of animal Science 99(3):83-84 (Abstract 155).

    2021 - View publication details

  2. Oral presentation- no abstract

    2021 - View publication details

  3. Palin, M.F., Lapointe, J., Gariépy, C., Beaudry, D., Kalbe, C. (2020). Characterisation of intracellular molecular mechanisms modulated by carnosine in porcine myoblasts under basal and oxidative stress conditions. PLoS ONE, [online] 15(9 September), http://dx.doi.org/10.1371/journal.pone.0239496

    2020 - View publication details

  4. The study objective was to determine the effects of hyperprolactinemia on the mammary parenchymal
    transcriptome in late-pregnant gilts. Gilts were divided into 3 groups on day 90 of gestation to receive IM injections of 1) canola oil (CTL, n = 18) until day 109 of gestation, 2) domperidone (dopamine antagonist)
    until day 96 (T7, n = 17) or, 3) domperidone until day 109 (T20, n = 17). Mammary glands were collected
    on day 110 and parenchymal tissue was sampled for transcriptomic analyses. Total RNA was isolated from
    6 CTL and 6 T20 gilts for microarray analysis. The GeneChip® Porcine Gene 1.0 ST Array (19,202 genes)
    was used for hybridization. Array quality control, data normalization and expression level analyses were
    performed with the Affymetrix Expression Console and Transcriptome Analysis Console (TAC) software. Using a threshold cut-off of 1.5 fold (P < 0.05), a total of 313 upregulated and 480 downregulated gene transcripts were identified in T20 vs CTL gilts. A qPCR validation analysis of selected upregulated (n = 13) and downregulated (n = 13) genes was conducted on all animals (CTL, T7, T20). The MIXED procedure of SAS was used for statistical analyses. All selected genes were validated for the CTL vs T20 comparison (P < 0.01). Only 4 selected genes (CAMK1G, COL9A1, P2RX7, TDRD1) were downregulated in the T7 treatment (vs CTL, P < 0.05). Functional analyses of differentially expressed genes were performed using the PANTHER classification system. The top upregulated Biological Process enriched GO terms were Inflammatory Response (GO:0006954) and Response to Lipid (GO:0033993). The Positive Regulation of Cell Population Proliferation (GO:0008284) and Regulation of Cellular Catabolic Process (GO:0031329) GO terms were identified for downregulated genes. Results suggest that a sustained hyperprolactinemia during latepregnancy (T20 treatment) may increase mammary inflammatory response and reduce cell proliferation.

    2020 - View publication details

  5. Caron, A., Palin, M.F., Hovey, R.C., Cohen, J., Laforest, J.P., Farmer, C. (2020). Effects of sustained hyperprolactinemia in late gestation on mammary development of gilts. Domestic Animal Endocrinology, [online] 72 http://dx.doi.org/10.1016/j.domaniend.2019.106408

    2020 - View publication details

  6. Palin, M.F., Lapointe, J., Gariépy, C., Beaudry, D. and Kalbe, C. (2019) Carnosine prevents oxidative damage in myoblast cells derived from porcine skeletal muscle (Invited keynote speaker) Journal of Animal Science, Vol 97, Suppl. 3: p59 (Abstract 57). https://doi.org/10.1093/jas/skz258.122.

    2019 - View publication details

  7. Caron, A., Hovey, R.C., Palin, M.F., Cohen, J.R., Laforest, J.P. and Farmer, C. (2019) Effects of sustained hyperprolactinemia in late gestation on mammary development of gilts. Journal of Animal Science, Vol. 97, Suppl. 3: p289, (Abstract PSI-7). https://doi.org/10.1093/jas/skz258.584

    2019 - View publication details

  8. Palin, M.F., Gariépy, C., Beaudry, D., Lapointe, J. and Kalbe, C. (2019) Protective effects of carnosine on hydrogen peroxide–induced oxidative stress in myoblast cells derived from porcine skeletal muscle. 65th International Congress of Meat Science and Technology 2019 in Potsdam/Berlin, Germany 04– 09 August 2019. (Abstract and Poster)

    2019 - View publication details

  9. Li Y.Y., Palin M.F., Yaylayan V., Sullivan B., Fortin, F., Cliche, S. and Gariépy, C. (2019) Carnosine reduction of AGEs (CML) formation during digestion of meat. 65th International Congress of Meat Science and Technology 2019 in Potsdam/Berlin, Germany 04– 09 August 2019. (Abstract and Poster)

    2019 - View publication details

  10. Duarte, C.R.A., Farmer, C., Palin, M.F. (2019). Body condition of late pregnant gilts affects the expression of selected adipokines and their receptors in mammary fat and backfat tissues. Journal of Animal Science, [online] 97(1), 220-230. http://dx.doi.org/10.1093/jas/sky391

    2019 - View publication details

  11. Palin, M.F., Gariépy, C., Beaudry, D., Lapointe, J. and Kalbe, C. (2018) Carnosine prevents oxidative damage in porcine muscle cells. Canadian Meat Council's 98th Annual Conference, May 29-31, 2018, Montreal, Quebec.

    2018 - View publication details

  12. Li, Y., Palin, M.F., Yaylayan, V., Sullivan, B., Fortin, F., Binnie, M.A., Young, M., Cliche, S. and Gariépy, C. (2018) Functional effects of carnosine during in vitro digestion of pork with different fat content and cooking conditions. Canadian Meat Council's 98th Annual Conference, May 29-31, 2018, Montreal, Quebec. (Abstract and poster)

    2018 - View publication details

  13. Gariépy, C. and Palin, M.F. (2018) Carnosine: The meat’s best-kept secret. Canadian Meat Council's 98th Annual Conference, May 29-31, 2018, Montreal, Quebec. (Invited presentation and abstract)

    2018 - View publication details

  14. Facebook: Another tool to help ensure quality pork! M.F. Palin and C. Gariépy. Work on pig muscle carnosine that was posted on the Agriculture and Agri-Food Facebook page. May 4th 2018.

    2018 - View publication details

  15. Des chercheurs d’Agriculture et Agroalimentaire Canada découvrent une molécule qui améliore la qualité de la viande de porc/Agriculture and Agri-Food Canada Researchers Find Pork Molecule Boosts Meat Quality. Palin, M.F. and Gariépy, C. April 18th 2018.

    http://www.agr.gc.ca/eng/science-and-innovation/research-centres-and-collections/ quebec/sherbrooke-research-and-development-centre/researchers-find-pork-molecule-boosts-meat-quality/?id=1523983629242

    2018 - View publication details

  16. Sullivan, B., Gariépy, G., Binnie, MS, Fortin, F., Goddard, H., Palin, M.F., Pomar, C. and Young, M. Increasing Canadian pork consumption, market share and competitiveness through enhanced nutritional values and overall quality with a functional molecule in pork meat. In Swine Innovation Porc 2017-2018 Final Report. pp. 50-51.

    2018 - View publication details

  17. Beaulieu, D., Farmer, C., Brown, J., Lapointe, J. and Palin, M.F. Increasing sow milk yield and piglet growth via low-cost feeding and management strategies during gestation and/or lactation. In Swine Innovation Porc 2017-2018 Final Report. pp. 14-15.

    2018 - View publication details

  18. Weekly Science Story: Une molécule du porc améliore les avantages nutritionnels/Pork Molecule Improves Nutritional Benefits. February 28th 2018. Claude Gariépy, Simon Cliche, Marie-France Palin, Joël D'Astous-Pagé, Danièle Beaudry, Steve Méthot.
    https://collab.agr.gc.ca/br-dg/stb-dgst/SitePages/Weekly%20Science%20Stories.aspx

    2018 - View publication details

  19. Palin, M.F., Farmer, C., Duarte, C.R.A. (2017). Triennial lactation symposium/bolfa: Adipokines affect mammary growth and function in farm animals. Journal of Animal Science, [online] 95(12), 5689-5700. http://dx.doi.org/10.2527/jas2017.1777

    2017 - View publication details

  20. D'Astous-Pagé, J., Gariépy, C., Blouin, R., Cliche, S., Méthot, S., Sullivan, B., Fortin, F., Palin, M.F. (2017). Identification of single nucleotide polymorphisms in carnosine-related genes and effects of genotypes on pork meat quality attributes. Meat Science, [online] 134 54-60. http://dx.doi.org/10.1016/j.meatsci.2017.07.019

    2017 - View publication details

Research facility

2000 College Street
Sherbrooke, QC J1M 0C8
Canada

Affiliations

  • Adjunct professor at the biology department, Sherbrooke University, Sherbrooke, QC.

  • Member of the Genetic Committee of the Canadian Centre for Swine Improvement (CCSI).

  • Member of the Research and Development Genetic Forum, “Centre de Développement du Porc du Québec (CDPQ)”.

Language

English
French