Lone Buchwaldt, Ph.D.

Research Scientist

Plant pathologist with expertise in plant pathogens of agricultural crops with focus on canola, soya bean and lentil.
Identification of new sources of disease resistant germplasm.
Genomic mapping disease resistance loci and linked molecular markers.
Gene’s contribution to disease resistance by cloning and plant transformation.
Development of pre-breeding lines combining disease resistance, agronomic traits and seed quality.
Characterization of genomic diversity and aggressiveness in pathogen populations with focus on Sclerotinia, Colletotrichum and Ascochyta species.
Epidemiology and decision support systems for fungicide application.

Current research and/or projects

Development of canola pre-breeding lines combining resistance to the fungal pathogen, Sclerotinia sclerotiorum with suitable agronomic traits and seed quality.

Characterization of the Sclerotinia sclerotiorum population in western Canada for genomic diversity and aggressiveness on canola with quantitative resistance.

Cloning and transformation of defense genes to demonstrate their contribution to resistance against Sclerotinia resistance in canola.

Mapping of quantitative trait loci conferring resistance to Sclerotinia sclerotiorum in soybean for the Prairie Provinces.

Research and/or project statements

Publications

Derbyshire, M.C., Denton-Giles, M., Hane, J.K., Chang, S., Mousavi-Derzmahalleh, M., Raffaele, S., Buchwaldt, L., Kamphuis, L.G. 2019. A whole genome scan of SNP data suggests a lack of abundant hard selective sweeps in the genome of the broad host range plant pathogenic fungus Sclerotinia sclerotiorum. PLoS ONE 14(3): e0214201. https://doi.org/10.1371

Buchwaldt, L., Dzananovic, E., Durkin, J. 2018. Lentil anthracnose: epidemiology, fungicide decision support system, resistance, and pathogen races. Can J Plant Pathol. Vol 40 (2). https://doi.org/10.1080/07060661.2018.1441185.

Denton-Giles, M., Derbyshire, M.C, Khentry, Y., Buchwaldt, L., Kamphuis L.G. 2018. Partial stem resistance in Brassica napus to highly aggressive and genetically diverse Sclerotinia sclerotiorum isolates from Australia. Can. J Plant Pathol. 40: 551-561. https://doi.org/10.1080/07060661.2018.1516699.

Gyawali, S., Harrington, M., Durkin, J., Horner, K., Parkin, I.A.P., Hegedus, D.D., Bekkaoui, D., Buchwaldt, L. 2016. Microsatellite markers used for genome-wide association mapping of partial resistance to Sclerotinia sclerotiorum in a world collection of Brassica napus. Mol Breeding (2016) 36:72. DOI 10.1007/s11032-016-0496-5.

Durkin, J.M.H., Bissett, J., Pahlavani, M., Mooney, B., Buchwaldt, L. 2015. IGS minisatellites useful for race differentiation in Colletotrichum lentis and a likely site of small RNA synthesis affecting pathogenicity. PLOSone, 10(9): e0137398. doi:10.1371/journal. pone.0137398.

Shaikh, R., Diederichsen, A., Harrington, M., Adam, J., Conner, R.L., Buchwaldt, L. 2013. New sources of resistance to Colletotrichum truncatum race Ct0 and Ct1 in Lens culinaris subsp. culinaris obtained by single plant selection in germplasm accessions. Genetic Resources and Crop Evolution, 60(1), pp. 193-201. doi : 10.1007/s10722-012-9825-7

Gyawali, S., Hegedus, D.D., Parkin, I.A.P., Poon, J., Higgins, E.E., Horner, K., Bekkaoui, D.R., Coutu, C., Buchwaldt, L. 2013. Genetic diversity and population structure in a world collection of Brassica napus accessions with emphasis on those from South Korea, Japan and Pakistan. Crop Science, 53(4), pp. 1537-1545. doi : 10.2135/cropsci2012.10.0614

Buchwaldt, L., Shaikh, R., Adam, J., Tullu, A.D., Slinkard, A.E. 2013. Recessive and dominant genes confer resistance to Colletotrichum truncatum in cultivated lentil. Canadian Journal of Plant Pathology, 35(2), pp. 222-231. doi : 10.1080/07060661.2013.768296

Buchwaldt L. Lentil anthracnose. 2011. In: Compendium of Lentil and Chickpea Diseases. Eds. F. Muehlbauer and W. Chen. APS Press. The American Phytopathological Society. APS Press, 165 pages. Page 31-34.

Bashi, Z.D., Hegedus, D.D., Buchwaldt, L., and Rimmer, S.R., Borhan, M.H. 2010. Expression and regulation of Sclerotinia sclerotiorum necrosis and ethylene inducing peptides (NEPs). Molecular Plant Pathology, 11(1), pp. 43-53. doi : 10.1111/j.1364-3703.2009.00571.

Chen, W., Basandrai, A.K., Basandrai, D., Banniza, S., Bayaa, B., Buchwaldt, L., Davidson, J., Larsen, R.J., Rubiales, D., Taylor, P.W.J. 2009. Lentil diseases and their management. In Erskine, W., Muehlbauer, F.J., Sarker, A. and Sharma, B. (Eds) - The Lentil: Botany, Production and Uses, CABI Publishing, p. 262-281.

Gossen, B.D., Anderson, K.L., Buchwaldt, L. 2009. Host specificity of Colletotrichum truncatum from lentil. Canadian Journal of Plant Pathology, 31(1), p. 65-73. doi : 10.1080/07060660909507573.

Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Brkic, M., Bekkaoui, D.R., Hegedus, D.D. 2009. Differential expression of duplicated peroxidase genes in the allotetraploid Brassica napus. Plant Physiology and Biochemistry, 47(7), p. 653-656. doi : 10.1016/j.plaphy.2009.03.002

Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., Hegedus, D.D. 2009. Patterns of differential gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum. Molecular Plant Pathology, 10(5), p. 635-649. doi : 10.1111/j.1364-3703.2009.00558.

Rimmer, S.R. ,Schattuck, V.I., and Buchwaldt, L. Editors. 2007. Compendium on Brassica Crop Diseases. American Phytopathological Society. APS Press,117 pp.

Professional activities / interests

President of the Canadian Phytopathological Society 2021-2022.

Associated Editor for the Canadian Journal of Plant Pathology.

Lecture at University of Saskatchewan, Department of Plant Science. Course: Global Plant Genetic Resources.

Education and awards

Ph.D. Royal Veterinarian and Agricultural University, Copenhagen, Denmark.

International experience and/or work

Meeting organizer

15th International Rapeseed Congress. 2019, Berlin, Germany.

14th International Rapeseed Congress. 2015, Saskatoon, Canada.

Speaker

Rapeseed Conference. Berlin, Germany. 2019.

7th International Food Legume Research Conference. 2018. Marrakesh, Morocco. Epidemiology, fungicide control, and host resistance for management of lentil anthracnose in Canada.

Curtin University. Perth, Australia. 2017. Managing sclerotinia in canola.

University of Melbourne. Victoria, Australia. 2017. Sclerotinia research in Canada.

14th International Rapeseed Congress. 2015. Saskatoon, Canada. Mapping QTLs in Brassica napus providing resistance to the Sclerotinia sclerotiorum.

17th International Plant Protection Congress. 2015. Berlin, Germany. Utilization of quantitative resistance to the fungal pathogen Sclerotinia sclerotiorum in canola (Brassica napus).

USDA National Sclerotinia Initiative. 2013, Minneapolis, USA. Resistance to Sclerotinia sclerotiorum in Brassica napus.

3rd Ascochyta Workshop. 2012, Cordoba, Spain. Mapping of Ascochyta rabiei resistance QTL in chickpea.

13th International Rapeseed Conference. 2011, Prague, Cech Republic. Sclerotinia stem rot resistance in Brassica napus Zhongyou 821.

Key publications

Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Dow Agro Science, Saskatoon, SK, Canada, January 28, 2013. (Presentation)
2013 - View publication details
Buchwaldt, L., Hegedus, D.D., Lydiate, D.J., Parkin, I.A.P., Fu, F., Garg, H., Gyawali, S., and Rimmer, S.R. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", National Sclerotinia Initiative 9th Annual Meeting, Minneapolis, MN, USA, January 23-25, 2013.
2013 - View publication details
Buchwaldt, L., Fu, F., Gyawali, S., Hegedus, D.D., and Parkin, I.A.P. (2013). "Towards identification of quantitative loci for sclerotinia resistance in Brassica napus germplasm and the underlying defense genes.", GCIRC Technical Meeting, Geneva, Switzerland, April 28- May 1, 2013. (Abstract)
2013 - View publication details
Gyawali, S., Hegedus, D.D., Parkin, I.A.P., Poon, J., Higgins, E., Horner, K., Bekkaoui, D., Coutu, C., Buchwaldt, L. (2013). Genetic diversity and population structure in a world collection of Brassica napus accessions with emphasis on South Korea, Japan, and Pakistan. Crop Science, [online] 53(4), 1537-1545. http://dx.doi.org/10.2135/cropsci2012.10.0614
2013 - View publication details
Buchwaldt, L., Shaikh, R., Adam, J., Tullu, A., Slinkard, A.E. (2013). Recessive and dominant genes confer resistance to Colletotrichum truncatum in cultivated lentil. Canadian Journal of Plant Pathology, [online] 35(2), 222-231. http://dx.doi.org/10.1080/07060661.2013.768296
2013 - View publication details
Shaikh, R., Diederichsen, A., Harrington, M., Adam, J., Conner, R.L., Buchwaldt, L. (2013). New sources of resistance to Colletotrichum truncatum race Ct0 and Ct1 in Lens culinaris Medikus subsp. culinaris obtained by single plant selection in germplasm accessions. Genetic Resources and Crop Evolution, [online] 60(1), 193-201. http://dx.doi.org/10.1007/s10722-012-9825-7
2013 - View publication details
Buchwaldt, L., Kishore, G.K., Prabhakaran, R., Fu, F., Booker, H.M., Madrid, E.H., Millán, T., Gil, J., Rubin, J., and Sharpe, A.G. (2012). "Status and prospects of controlling ascochyta blight in chickpea by genetic resistance.", 9th Canadian Pulse Crop Research Workshop, Niagara Falls, ON, Canada, November 6-9, 2012. (Abstract)
2012 - View publication details
Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2012). "Characterization of S. sclerotiorum clones in western Canada for pathogenicity and genomic relatedness.", Canola Cluster update, Saskatoon, SK, Canada, March 21, 2012.
2012 - View publication details
Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2012). "A genomics approach to sclerotinia resistance in Brassica napus. SaskCanola 20th Anniversary Research Summary."
2012 - View publication details
Buchwaldt, L., Lydiate, D.J., Yu, F.Q., Fu, F., Hegedus, D.D., Gyawali, S., Garg, H., and Rimmer, S.R. (2012). "Advances in sclerotinia resistance research.", Canola Industry Meeting, Garden Inn Hotel, Saskatoon, SK, Canada, December 5, 2012. (Presentation)
2012 - View publication details
Simmonds, D.H., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Zhang, Y., Calla, B., Neece, D.J., and Clough, S.J. (2012). "Identification of candidate defence-related genes to Sclerotinia sclerotiorum in soybean; functional analysis in soybean, Arabidopsis, and Nicotiana benthamiana.", 14th Cellular and Molecular Biology of the Soybean Conference, Des Moines, Iowa, USA, August 12-15, 2012, Number 104. (Abstract)
2012 - View publication details
Buchwaldt, L., Shaikh, R., Diederichsen, A., Harrington, M., Adam, J., and Conner, R.L. (2012). "Sources of resistance to Colletotrichum truncatum in lentil obtained by cycles of single plant selection in diverse germplasm.", 9th Canadian Pulse Crop Research Workshop, Niagara Falls, ON, Canada, November 6-9, 2012. (Abstract)
2012 - View publication details
Simmonds, D.H., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Zhang, Y., Calla, B., Neece, D.J., and Clough, S.J. (2012). "Identification of candidate defence-related genes to Sclerotinia sclerotiorum in soybean; functional analysis in soybean, Arabidopsis, and Nicotiana benthamiana.", 6th International Conference on Legume Genetics and Genomics, Hyderabad, India, October 2-7, 2012, Number P-PAD30. (Abstract)
2012 - View publication details
Turkington, T.K., Kutcher, H.R., Jurke, C., Buchwaldt, L., and McLaren, D.L. (2012). "Managing sclerotinia stem rot of canola.", Webinar, Canola Council of Canada and canola grower’s associations (ACPC/SC/MCGA), Canada, June 28, 2012. (Presentation)
2012 - View publication details
Hegedus, D.D., Buchwaldt, L., and Parkin, I.A.P. (2012). "Development of sclerotinia resistant Brassica napus lines and molecular markers for marker-assisted breeding.", Canola Cluster update, Saskatoon, SK, Canada, March 21, 2012.
2012 - View publication details
Buchwaldt, L., Lydiate, D.J., Yu, F.Q., Hegedus, D.D., and Rimmer, S.R. (2011). "QTLs contributing to sclerotinia stem rot resistance in Brassica napus Zhongyou 821.", 13th International Rapeseed Congress, Prague, Czech Republic, June 5-9, 2011.
2011 - View publication details
Buchwaldt, L. (2011). "Utilization of sclerotinia resistance in canola from Asia.", Crop Protection Field Tour, Saskatoon Research Farm, Saskatoon, SK, Canada, July 20, 2011. (Presentation)
2011 - View publication details
Buchwaldt, L. (2011 Internal). Identification of DNA markers linked to QTLs for Sclerotinia sclerotiorum resistance in spring and winter types of Brassica napus. Final report and oral presentation to Bayer CropScience, Dow AgroScoence, KWS and DL Seeds. (Report)
2011 - View publication details
Gyawali, S., Buchwaldt, L., Harrington, M., Parkin, I.A.P., Bekkaoui, D.R., Horner, K., Higgins, E.E., and Hegedus, D.D. (2011). "Association mapping of stem rot resistance in a world collection of Brassica napus.", American Phytopathological Society (APS) 2011 Joint Annual Meeting & International Association for the Plant Protection Sciences (IPPC), Honolulu, HI, USA, August 6-10, 2011.
2011 - View publication details
Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2011). "Sclerotinia resistance in B. napus – an update.", Cargill, Aberdeen, SK, Canada, November 23, 2011. (Presentation)
2011 - View publication details