Lone Buchwaldt, Ph.D.

Research Scientist

Plant pathologist with expertise in plant pathogens of agricultural crops with focus on canola, soya bean and lentil.
Identification of new sources of disease resistant germplasm.
Genomic mapping disease resistance loci and linked molecular markers.
Gene’s contribution to disease resistance by cloning and plant transformation.
Development of pre-breeding lines combining disease resistance, agronomic traits and seed quality.
Characterization of genomic diversity and aggressiveness in pathogen populations with focus on Sclerotinia, Colletotrichum and Ascochyta species.
Epidemiology and decision support systems for fungicide application.

Current research and/or projects

Development of canola pre-breeding lines combining resistance to the fungal pathogen, Sclerotinia sclerotiorum with suitable agronomic traits and seed quality.

Characterization of the Sclerotinia sclerotiorum population in western Canada for genomic diversity and aggressiveness on canola with quantitative resistance.

Cloning and transformation of defense genes to demonstrate their contribution to resistance against Sclerotinia resistance in canola.

Mapping of quantitative trait loci conferring resistance to Sclerotinia sclerotiorum in soybean for the Prairie Provinces.

Research and/or project statements

Publications

Derbyshire, M.C., Denton-Giles, M., Hane, J.K., Chang, S., Mousavi-Derzmahalleh, M., Raffaele, S., Buchwaldt, L., Kamphuis, L.G. 2019. A whole genome scan of SNP data suggests a lack of abundant hard selective sweeps in the genome of the broad host range plant pathogenic fungus Sclerotinia sclerotiorum. PLoS ONE 14(3): e0214201. https://doi.org/10.1371

Buchwaldt, L., Dzananovic, E., Durkin, J. 2018. Lentil anthracnose: epidemiology, fungicide decision support system, resistance, and pathogen races. Can J Plant Pathol. Vol 40 (2). https://doi.org/10.1080/07060661.2018.1441185.

Denton-Giles, M., Derbyshire, M.C, Khentry, Y., Buchwaldt, L., Kamphuis L.G. 2018. Partial stem resistance in Brassica napus to highly aggressive and genetically diverse Sclerotinia sclerotiorum isolates from Australia. Can. J Plant Pathol. 40: 551-561. https://doi.org/10.1080/07060661.2018.1516699.

Gyawali, S., Harrington, M., Durkin, J., Horner, K., Parkin, I.A.P., Hegedus, D.D., Bekkaoui, D., Buchwaldt, L. 2016. Microsatellite markers used for genome-wide association mapping of partial resistance to Sclerotinia sclerotiorum in a world collection of Brassica napus. Mol Breeding (2016) 36:72. DOI 10.1007/s11032-016-0496-5.

Durkin, J.M.H., Bissett, J., Pahlavani, M., Mooney, B., Buchwaldt, L. 2015. IGS minisatellites useful for race differentiation in Colletotrichum lentis and a likely site of small RNA synthesis affecting pathogenicity. PLOSone, 10(9): e0137398. doi:10.1371/journal. pone.0137398.

Shaikh, R., Diederichsen, A., Harrington, M., Adam, J., Conner, R.L., Buchwaldt, L. 2013. New sources of resistance to Colletotrichum truncatum race Ct0 and Ct1 in Lens culinaris subsp. culinaris obtained by single plant selection in germplasm accessions. Genetic Resources and Crop Evolution, 60(1), pp. 193-201. doi : 10.1007/s10722-012-9825-7

Gyawali, S., Hegedus, D.D., Parkin, I.A.P., Poon, J., Higgins, E.E., Horner, K., Bekkaoui, D.R., Coutu, C., Buchwaldt, L. 2013. Genetic diversity and population structure in a world collection of Brassica napus accessions with emphasis on those from South Korea, Japan and Pakistan. Crop Science, 53(4), pp. 1537-1545. doi : 10.2135/cropsci2012.10.0614

Buchwaldt, L., Shaikh, R., Adam, J., Tullu, A.D., Slinkard, A.E. 2013. Recessive and dominant genes confer resistance to Colletotrichum truncatum in cultivated lentil. Canadian Journal of Plant Pathology, 35(2), pp. 222-231. doi : 10.1080/07060661.2013.768296

Buchwaldt L. Lentil anthracnose. 2011. In: Compendium of Lentil and Chickpea Diseases. Eds. F. Muehlbauer and W. Chen. APS Press. The American Phytopathological Society. APS Press, 165 pages. Page 31-34.

Bashi, Z.D., Hegedus, D.D., Buchwaldt, L., and Rimmer, S.R., Borhan, M.H. 2010. Expression and regulation of Sclerotinia sclerotiorum necrosis and ethylene inducing peptides (NEPs). Molecular Plant Pathology, 11(1), pp. 43-53. doi : 10.1111/j.1364-3703.2009.00571.

Chen, W., Basandrai, A.K., Basandrai, D., Banniza, S., Bayaa, B., Buchwaldt, L., Davidson, J., Larsen, R.J., Rubiales, D., Taylor, P.W.J. 2009. Lentil diseases and their management. In Erskine, W., Muehlbauer, F.J., Sarker, A. and Sharma, B. (Eds) - The Lentil: Botany, Production and Uses, CABI Publishing, p. 262-281.

Gossen, B.D., Anderson, K.L., Buchwaldt, L. 2009. Host specificity of Colletotrichum truncatum from lentil. Canadian Journal of Plant Pathology, 31(1), p. 65-73. doi : 10.1080/07060660909507573.

Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Brkic, M., Bekkaoui, D.R., Hegedus, D.D. 2009. Differential expression of duplicated peroxidase genes in the allotetraploid Brassica napus. Plant Physiology and Biochemistry, 47(7), p. 653-656. doi : 10.1016/j.plaphy.2009.03.002

Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., Hegedus, D.D. 2009. Patterns of differential gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum. Molecular Plant Pathology, 10(5), p. 635-649. doi : 10.1111/j.1364-3703.2009.00558.

Rimmer, S.R. ,Schattuck, V.I., and Buchwaldt, L. Editors. 2007. Compendium on Brassica Crop Diseases. American Phytopathological Society. APS Press,117 pp.

Professional activities / interests

President of the Canadian Phytopathological Society 2021-2022.

Associated Editor for the Canadian Journal of Plant Pathology.

Lecture at University of Saskatchewan, Department of Plant Science. Course: Global Plant Genetic Resources.

Education and awards

Ph.D. Royal Veterinarian and Agricultural University, Copenhagen, Denmark.

International experience and/or work

Meeting organizer

15th International Rapeseed Congress. 2019, Berlin, Germany.

14th International Rapeseed Congress. 2015, Saskatoon, Canada.

Speaker

Rapeseed Conference. Berlin, Germany. 2019.

7th International Food Legume Research Conference. 2018. Marrakesh, Morocco. Epidemiology, fungicide control, and host resistance for management of lentil anthracnose in Canada.

Curtin University. Perth, Australia. 2017. Managing sclerotinia in canola.

University of Melbourne. Victoria, Australia. 2017. Sclerotinia research in Canada.

14th International Rapeseed Congress. 2015. Saskatoon, Canada. Mapping QTLs in Brassica napus providing resistance to the Sclerotinia sclerotiorum.

17th International Plant Protection Congress. 2015. Berlin, Germany. Utilization of quantitative resistance to the fungal pathogen Sclerotinia sclerotiorum in canola (Brassica napus).

USDA National Sclerotinia Initiative. 2013, Minneapolis, USA. Resistance to Sclerotinia sclerotiorum in Brassica napus.

3rd Ascochyta Workshop. 2012, Cordoba, Spain. Mapping of Ascochyta rabiei resistance QTL in chickpea.

13th International Rapeseed Conference. 2011, Prague, Cech Republic. Sclerotinia stem rot resistance in Brassica napus Zhongyou 821.

Key publications

Gyawali, S., Harrington, M., Durkin, J., Horner, K., Parkin, I.A.P., Hegedus, D.D., Bekkaoui, D., Buchwaldt, L. (2016). Microsatellite markers used for genome-wide association mapping of partial resistance to Sclerotinia sclerotiorum in a world collection of Brassica napus. Molecular Breeding, [online] 36(6), http://dx.doi.org/10.1007/s11032-016-0496-5
2016 - View publication details
Fu, F., Rimmer, S.R., Lydiate, D.J., Hegedus, D.D., Parkin, I.A.P., Adam, J., Harrington, M., McGregor, L., and Buchwaldt, L. (2015). "Mapping of quantitative trait loci in Brassica napus lines from Pakistan and China providing resistance to the Sclerotinia sclerotiorum.", 14th International Rapeseed Congress, Saskatoon, SK, Canada, July 5-9, 2015. (Abstract)
2015 - View publication details
Blahut-Beatty, L., Koziol, L., Calla, B., Buchwaldt, L., Neece, D.J., Cough, S.J., and Simmonds, D.H. (2015). "Strategies to identify functionally significant defense genes against Sclerotinia sclerotiorum.", USDA-ARS National Sclerotinia Initiative. 13th Annual Meeting, Bloomington, MN, USA, January 21-23, 2015, Number 9, pp. 30. (Abstract)
2015 - View publication details
Durkin, J.M.H., Bissett, J.D., Song, J.-R., Pahlavani, M., Mooney, B.G., and Buchwaldt, L. (2015). "Minisatellites in IGS useful for race differentiation in Colletotrichum of lentil and a likely site of small RNA synthesis affecting pathogenicity.", 28th Fungal Genetics Conference, Pacific Grove, CA, USA, March 17-22, 2015, Abstract No. 578; Selected oral presentation, pp. 293. (Abstract)
2015 - View publication details
Puri, K.D., Garg, H., Durkin, J.M.H., Adam, J., Harrington, M., Liabeuf, D., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2015). "Characterization of the Sclerotinia sclerotiorum population from canola in western Canada needed for selection of partially resistant Brassica napus germplasm.", 14th International Rapeseed Congress, Saskatoon, SK, Canada, July 5-9, 2015. (Abstract)
2015 - View publication details
Blahut-Beatty, L., Koziol, L., Calla, B., Buchwaldt, L., Neece, D.J., Clough, S.J., and Simmonds, D.H. (2015). "Strategies to identify functionally significant defense genes against Sclerotinia sclerotiorum.", USDA-ARS National Sclerotinia Initiative. 12th Annual Meeting, Bloomington, MN, USA, January 22-24, 2014, pp. 32.
2015 - View publication details
Durkin, J., Bissett, J., Pahlavani, M., Mooney, B., Buchwaldt, L. (2015). IGS minisatellites useful for race differentiation in Colletotrichum lentis and a likely site of small RNA synthesis affecting pathogenicity. PLoS ONE, [online] 10(9), http://dx.doi.org/10.1371/journal.pone.0137398
2015 - View publication details
Buchwaldt, L., Hegedus, D.D., Parkin, I.A.P., Vail, S.L., Fu, F., Garg, H., Gyawali, S., and Puri, K.D. (2014). "Dissecting quantitative resistance to sclerotinia in Brassica napus.", Applying Genomics to Canola Improvement Workshop, Saskatoon, SK, Canada, December 4, 2014, Invited oral presentation. (Abstract)
2014 - View publication details
Simmonds, D.H., Calla, B., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Neece, D.J., and Clough, S.J. (2014). "Gene expression studies to identify and verify the function of soybean defense genes against Sclerotinia sclerotiorum.", 15th Biennial Conference Molecular and Cellular Biology of the Soybean, Minneapolis, Minnesota, USA, August 3-6, 2014, pp. 44.
2014 - View publication details
Durkin, J.M.H., Bissett, J.D., Song, R., Pahlavani, H., Mooney, B.G., and Buchwaldt, L. (2014). "A change in the Colletotrichum truncatum population signals a need for new resistance in lentil.", 6th International Food Legumes Research Conference, Saskatoon, SK, Canada, July 7-11, 2014, Number 166, pp. 168. (Abstract)
2014 - View publication details
Prabhakaran, R., Varshney, R.K., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2014). "Resistance in chickpea to Ascochyta rabiei is conferred by genes encoding pathogen recognition and other genes in basic defense pathways.", 6th International Food Legumes Research Conference, Saskatoon, SK, Canada, July 7-11, 2014, Number 20, pp. 94. (Abstract)
2014 - View publication details
Turkington, T.K., Buchwaldt, L., Orchard, D., Strelkov, S.E., Ziesman, B.R., and Lange, R. (2014). "Assessing risk and spray timing for sclerotinia stem rot.", Daily interactive workshops, 2014 CanoLAB training event, Bolting and Beyond Session, Olds, Alberta, Canada, February 19-21, 2014. (Presentation)
2014 - View publication details
Simmonds, D.H., Calla, B., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Neece, D.J., and Clough, S.J. (2014). "Gene expression studies to identify and verify the function of soybean defense genes against Sclerotinia sclerotiorum.", 2nd Plant Genomics Congress, Europe, London, UK, May 11-14, 2014. (Poster)
2014 - View publication details
Puri, K.D., Garg, H., Durkin, J.M.H., Hegedus, D.D., Harrington, M., Adam, J., Liabeuf, D., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2014). "Genetic and pathogenic variability of Sclerotinia sclerotiorum from canola in Western Canada.", CPS Regional Meeting, Saskatchewan. SK, Canada. (Abstract)
2014 - View publication details
Bissett, J.D. and Buchwaldt, L. (2014). "Sequence repeat patterns in the IGS region distinguish genetically highly similar pathovars of Colletotrichum viciae on lentils.", 10th International Mycological Conference (IMC10), Bangkok, Thailand, August 3-8, 2014.
2014 - View publication details
Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Cargill, Aberdeen, SK, Canada, March 14, 2013. (Presentation)
2013 - View publication details
Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Dow Agro Science, Saskatoon, SK, Canada, January 28, 2013. (Presentation)
2013 - View publication details
Buchwaldt, L., Hegedus, D.D., Lydiate, D.J., Parkin, I.A.P., Fu, F., Garg, H., Gyawali, S., and Rimmer, S.R. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", National Sclerotinia Initiative 9th Annual Meeting, Minneapolis, MN, USA, January 23-25, 2013.
2013 - View publication details
Buchwaldt, L., Fu, F., Gyawali, S., Hegedus, D.D., and Parkin, I.A.P. (2013). "Towards identification of quantitative loci for sclerotinia resistance in Brassica napus germplasm and the underlying defense genes.", GCIRC Technical Meeting, Geneva, Switzerland, April 28- May 1, 2013. (Abstract)
2013 - View publication details
Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Bayer Crop Science, Saskatoon, SK, Canada, February 26, 2013. (Presentation)
2013 - View publication details