Izhar Khan, Ph.D.

Research Scientist

My research interests, in the field of Environmental Health, Biodiversity and Bioresources, are to develop rapid and simple state-of-the-art quantitative methods for rapid detection and identification of water- and foodborne human and animal pathogens for identification of sources of fecal pollution in various environmental niches. In addition, I have been working on taxonomic classification of bacteria such as genus Acinetobacter, Campylobacter and Arcobacter and study their pathogenicity and antibiotic resistance to human and animals using Next Generation Sequencing in order to provide insights into the genetic diversity and evolutionary relationship among species and strains.

Current research and/or projects

  • Impact of agricultural practices and environmental factors on the distribution and diversity of bacterial waterborne pathogens in agriculture watersheds
  • Development of state-of-art molecular tools for the rapid detection and identification of micro-aerophilic pathogens
  • Quantitative real-time PCR analysis for detection and quantification of human and animal bacterial and viral pathogens from various environmental sources
  • Molecular detection and characterization of emerging waterborne pathogens and microbial water quality indicators in agricultural and urban watersheds
  • Genotypic characterization and of pathogens by DNA sequencing, PFGE, RFLP analyses

Research and/or project statements

1. Strengthening the competitiveness and sustainability of the Canadian agriculture and agri-food sector

2. Promoting innovation and a healthy environment by focusing on following priorities to provide the best scientific information for regulatory and policy decisions:

       i).Enhance environmental performance of the Canadian agricultural system;

       ii). Improve environmental performance and sustainability of swine or dairy production systems;

       iii). Enhance the quality of food and the safety of the food system; iv) Improving the identification and understanding of crop pests and beneficial organisms;

       v) Reducing soil chemical, biological and physical degradation and maintaining soil health by improving farm and manure management practices; 

       vi). Identify the BMPs to mitigate the risk of water, soil and air contamination.

Education and awards

  • Ph.D. Justus-Liebig University Giessen, Germany
  • M.Sc. Agriculture University Faisalabad, Pakistan
  • D.V.M. Agriculture University Faisalabad, Pakistan

International experience and/or work

Physical Scientist, Environment Canada- Water Science and Technology- Microbiology, Canada Centre for Inland Waters (CCIW), Burlington, ON.  

NSERC Visiting Fellowship in Canadian Government Laboratories, Environment Canada- CCIW, Burlington, ON.                   

Research Consultant, Environment Canada, CCIW, Burlington, ON.

Research Scholar, University of Cincinnati, OH, U.S.A.

Research Assistant, Justus-Liebig University Giessen, Germany.

Veterinary Officer, Livestock and Poultry Dept. Balochistan, Pakistan

Key publications

  1. Edge, T.A., Hill, S., Khan, I.U.H., Gannon, V.P.J., Crane, C., Collins, H.M., Johnson, R., Topp, E., and Roberts, C. (2014). "Microbial source tracking of pathogen and fecal pollution in shellfish harvesting areas in Atlantic Canada.", XVIth International Congress of Virology - International Union of Microbiological Societies (IUMS 2014), July 27 - August 1, 2014, Montréal, Québec, Canada, July 27-August 1, 2014.

    2014 - View publication details

  2. Tambong, J.T., Adam, Z., Lewis, C.T., Lévesque, C.A., Chen, W., Khan, I.U.H., Bromfield, E.S.P., and Xu, R. (2014). "Comparative genomics of Pantoeastewartii subspecies and development of a TaqManReal-Time PCR assay for specific detection of P. stewartii subsp.stewartii.", 13th International Conference of Plant Pathogenic Bacteria, Shanghai, China, June 8-13, 2014, pp. 34. (Abstract)

    2014 - View publication details

  3. Brazeau, K., Cloutier, M., and Khan, I.U.H. (2014). "Development of quantitative real-time PCR-based assays for the detection and identification of human and animal viruses in environmental matrices.", Biotechnology Poster Competition, La Cité Collégiale, Ottawa, ON, Canada, April 26, 2014. (Poster)

    2014 - View publication details

  4. Mazzola, L.G., Khan, I.U.H., and Vierula, J. (2014). "Comparative analysis of three DNA extraction kits and PCR assays for purification and identification of fungal DNA from agricultural water.", Biology Poster Competition, Carleton University, Ottawa, ON, Canada, April 10, 2014. (Poster)

    2014 - View publication details

  5. Khan, I.U.H., Gannon, V., Jokinen, C.C., Kent, R., Koning, W., Lapen, D.R., Medeiros, D., Miller, J., Neumann, N.F., Phillips, R., Schreier, H., Topp, E., van Bochove, E., Wilkes, G., Edge, T.A. (2014). A national investigation of the prevalence and diversity of thermophilic Campylobacter species in agricultural watersheds in Canada. Water Research, [online] 61 243-252. http://dx.doi.org/10.1016/j.watres.2014.05.027

    2014 - View publication details

  6. Adam, Z., Tambong, J.T., Lewis, C.T., Lévesque, C.A., Chen, W., Bromfield, E.S.P., Khan, I.U.H., Xu, R. (2014). Draft genome sequence of Pantoea ananatis strain LMG 2665T, a bacterial pathogen of pineapple fruitlets. Genome Announcements, [online] 2(3), http://dx.doi.org/10.1128/genomeA.00489-14

    2014 - View publication details

  7. Adam, Z., Whiteduck-Léveillée, K., Cloutier, M., Chen, W., Lewis, C.T., Lévesque, C.A., Topp, E., Lapen, D.R., Tambong, J.T., Talbot, G., Khan, I.U.H. (2014). Draft genome sequences of two Arcobacter strains isolated from human feces. Genome Announcements, [online] 2(2), http://dx.doi.org/10.1128/genomeA.00113-14

    2014 - View publication details

  8. Wilkes, G., Brassard, J., Edge, T.A., Gannon, V., Gottschall, N., Jokinen, C.C., Jones, T.H., Khan, I.U.H., Marti, R., Sunohara, M.D., Topp, E., Lapen, D.R. (2014). Long-term monitoring of waterborne pathogens and microbial source tracking markers in paired agricultural watersheds under controlled and conventional tile drainage management. Applied and Environmental Microbiology, [online] 80(12), 3708-3720. http://dx.doi.org/10.1128/AEM.00254-14

    2014 - View publication details

  9. Adam, Z., Whiteduck-Leveillee, K., Cloutier, M., Tambong, J.T., Chen, W., Lewis, C.T., Lévesque, C.A., Topp, E., Lapen, D.R., Talbot, G., Khan, I.U.H. (2014). Draft genome sequences of three Arcobacter strains of pig and dairy cattle manure origin. Genome Announcements, [online] 2(3), http://dx.doi.org/10.1128/genomeA.00377-14

    2014 - View publication details

  10. Ryu, H., Elk, M., Khan, I.U.H., Harwood, V.J., Molina, M., Edge, T.A., Domingo, J.S. (2014). Comparison of two poultry litter qPCR assays targeting the 16S rRNA gene of Brevibacterium sp.. Water Research, [online] 48(1), 613-621. http://dx.doi.org/10.1016/j.watres.2013.10.015

    2014 - View publication details

  11. Kaneza, C.A., Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Identification and detection of Arcobacter spp. from water and fecal matter using genus- and species-specific PCR assays.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013. (Poster)

    2013 - View publication details

  12. Laprade, N., Whiteduck-Léveillée, J., Whiteduck-Leveillee, K., Cloutier, M., Tambong, J.T., Topp, E., Lapen, D.R., Wilkes, G.A., and Khan, I.U.H. (2013). "Detection of virulence, toxin and tet(O) resistant genes in thermophilic Campylobacter species using multiplex PCR assays.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.

    2013 - View publication details

  13. Whiteduck-Leveillee, J., Whiteduck-Leveillee, K., Kaneza, C.A., Cloutier, M., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Multiplex PCR assay for the detection and identification of virulent genes in Arcobacter species.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.

    2013 - View publication details

  14. Poulin, G., Laprade, N., and Khan, I.U.H. (2013). "Development and application of DNA-based real-time PCR assay for the rapid detection of Campylobacter coli in agricultural waters.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013.

    2013 - View publication details

  15. Léveillée, K., Laprade, N., Léveillée, W.J., Topp, E., Lapen, D.R., Tambong, J.T., Talbot, G., Wilkes, G.A., and Khan, I.U.H. (2013). "Antimicrobial resistance profiling of Arcobacter butzleri isolates from faecal sources and an agricultural watershed.", 63rd Annual Conference of the Canadian Society of Microbiologists, Carleton University, Ottawa, ON, Canada, June 17-20, 2013, Abstract AE87.

    2013 - View publication details

  16. Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Kaneza, C.A., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Development of genus-specific PCR assay for rapid identification of Arcobacter species: Optimization of PCR Primers and Conditions, and Restriction Pattern Analysis.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.

    2013 - View publication details

  17. Kaneza, C.A., Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Identification and detection of Arcobacter spp. From water and fecal matter using genus- and species-specific PCR assays.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013.

    2013 - View publication details

  18. Léveillée, W.J., Kaneza, C.A., Léveillée, K., Talbot, G., Topp, E., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Optimization of multiplex PCR assays for the detection of virulence genes in Arcobacter species isolated from fecal samples.", 63rd Annual Conference of the Canadian Society of Microbiologists, Carleton University, Ottawa, ON, Canada, June 17-20, 2013, Abstract AE86.

    2013 - View publication details

  19. Khan, I.U.H., Cloutier, M., Edge, T.A., Schellhorn, H.E., Gupta, R.S., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Wilkes, G.A., Nowak, E.K., and Hill, S. (2013). "A comparative study on the prevalence of thermophilic Campylobacter spp. In agricultural and recreational waters.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.

    2013 - View publication details

  20. Hamelin, M-A., Cloutier, M., and Khan, I.U.H. (2013). "Detection and identification of thermophilic Campylobacter species in agricultural watershed in Ontario.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013.

    2013 - View publication details

Research facility

960 Carling Avenue
Ottawa, ON K1A 0C6
Canada

Affiliations

  • Member, American Society for Microbiology (ASM)
  • Member, Canadian Society of Microbiologists (CSM)
  • Member, Canadian College of Microbiologists (CCM)
  • Member, Canadian Association on Water Quality (CAWQ)

Language

English

Other languages

Urdu, Punjabi, German