Guillaume J. Bilodeau

Image Bilodeau, Guillaume
Research Scientist

Canadian Food Inspection Agency (CFIA), Ottawa Plant Laboratory (Fallowfield) - Pathogen Identification Research. Plant Pathogens Identification Research Lab (PIRL). Plans, organizes, coordinates and conducts research and development of technologies for detection and identification of plant pests (fungi-oomycetes) of regulatory significance. Expertise: Fungal detection and genotyping, Phytophthora, Phytophthora ramorum, Verticillium, Real-Time PCR, Molecular biology, genomic, metagenomic.

Research and/or project statements

  • Detection and genotyping of of plant pathogens of regulatory concern
  • Evaluation of genomics for detection of of plant pathogens of regulatory concern

Professional activities / interests

  • Member of the American Phytopathology Society.
  • Member of the Canadian Phytopathological Society.
  • Member of the Quebec Society for Plant Protection.
  • Speaker at many conferences.
  • Supervision of graduate students and postdoctoral fellows.
  • Review of scientific texts and grant applications.

Education and awards

Education:

2008, Ph. D. degree in microbiology, Laval University, Québec, Québec, Canada

Thesis subject: Detection and genomic of Phytophthora ramorum causal agent of sudden oak death.

2003, M. sc. degree in microbiology, Laval University, Québec, Québec, Canada

2002, Bachelor's degree in microbiology, Laval University, Québec, Québec, Canada

Awards:

  • 2017 CFIA President's National Award, Exceptional Service Delivery. Biosurveillance of Alien Forest Enemies (bioSAFE).
  • 2016 Public Service Award of Excellence, Scientific contribution;
  • 2016 CFIA President's National Award. Dr. Brian Evans Award for partnership and team work.
  • CFIA Science Branch award 2016.
  • Natural Resources Canada Merit Award, Creativity and Innovation, 2008
  • CFS (Canadian Forest Service) Merit Awards 2007-2008

Key publications

2019

Tremblay, Émilie D. Duceppe, Marc-Olivier Thurston, Graham B. Gagnon, Marie-Claude Côté Marie-José and Guillaume J. Bilodeau 2019. High-resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive. Environmental DNA. 1:155–175 https://doi.org/10.1002/edn3.17.

 

Dale, Angela L., Feau, Nicolas, Everhart, Sydney E., Dhillon, Braham, Wong, Barbara, Sheppard, Julie, Bilodeau, Guillaume J., Brar, Avneet, Tabima, Javier F., Shen, Danyu, Brasier, Clive M., Tyler, Brett M., Grünwald, Niklaus J., and Richard C. Hamelin. 2019. Mitotic Recombination and Rapid Genome Evolution in the Invasive Forest Pathogen Phytophthora ramorum. mBio , 10,  (2),  e02452-18;  DOI: 10.1128/mBio.02452-18

 

Feau, Nicolas Ojeda, Dario I., Beauseigle, Stéphanie, Bilodeau, Guillaume J., Brar, Avneet, Cervantes-Arango, Sandra, Dale, Angela L., Dhillon, Braham, Hammett, Craig, Herath, Padmini Shamoun, Simon F., Tsui, Clement K.M., Tanguay, Philippe, and Richard C. Hamelin. 2019. Improved detection and identification of the sudden oak death pathogen Phytophthora ramorum and the Port Orford cedar root pathogen Phytophthora lateralis. Plant Pathology, 68, 878–888. https://doi.org/10.1111/ppa.13015.

 

Mbareche, Hamza, Veillette, Marc, Teerstra, Wieke Kegel, Willem Bilodeau Guillaume, Wösten Han, and Caroline Duchaine 2019. Fungal Cells Recovery From Air Samples: A Tale of Loss And Gain. Appl. Environ. Microbiol. Volume 85 Issue 9 e02941-18, DOI: 10.1128/AEM.02941-18

 

Tremblay, É.D.; Kimoto, T.; Bérubé, J.A.; Bilodeau, G.J. High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps. J. Fungi 2019, 5, 15.

 

2018

Tremblay, Emilie D., Duceppe, Marc-Olivier, Berube, Jean A., Kimoto, Troy, Lemieux, Claude and Guillaume J. Bilodeau. 2018. Screening for exotic forest pathogens to increase survey capacity using metagenomics. Phytopathology108 (12) 1509-1521.https://doi.org/10.1094/PHYTO-02-18-0028-R

 

Mbareche, Hamza, Marc Veillette, Guillaume J Bilodeau and Caroline Duchaine. 2018. Occupational Exposure to Fungal Bioaerosols at Dairy Farms using a Next-Generation Sequencing Survey. Science of the Total Environment. 663, 253-263. https://doi.org/10.1016/j.scitotenv.2018.10.345

 

Mbareche, Hamza, Marc Veillette, Guillaume Bilodeau, and Caroline Duchaine. 2018. Comparison of Three High Flow Rate Air Samplers and their Effect on Microbial Ecology Studies of Bioaerosols. Applied and Environmental Microbiology. 84 (23). DOI: · 10.1128/AEM.01589-18

 

Berube Jean A., Gagne Patrick N., Ponchart Julien P., Tremblay Émilie D. and Guillaume J. Bilodeau, 2018. Detection of Diplodia corticola spores in Ontario and Québec based on HighThroughput Sequencing (HTS) methods. Canadian Journal of Plant Pathology. 40 (3), P 378-386. https://doi.org/10.1080/07060661.2018.1498394

 

Roe, Amanda D., Torson, Alex S., Bilodeau, Guillaume, Bilodeau, Pierre, Blackburn, Gwylim S., Cui, Mingming, Cusson, Michel, Doucet, Daniel, Griess, Verena C., Lafond, Valentine, Paradis, Gregory, Porth, Ilga, Prunier, Julien, Srivastava, Vivek, Tremblay, Emilie, Uzunovic, Adnan, Yemshanov, Denys, and Hamelin, Richard C. 2018. Biosurveillance of Forest Insects.  Part I: Integration and application of genomic tools to the surveillance of non-native forest insects. Journal of Pest Science 92 (1), 51-70. https://doi.org/10.1007/s10340-018-1027-4.

 

Pierre Bilodeau, Amanda D. Roe, Guillaume Bilodeau, Gwylim S. Blackburn, Mingming Cui, Michel Cusson, Daniel Doucet, Verena C. Griess, Valentine M. A. Lafond, Chelsea Nilausen, Gregory Paradis, Ilga Porth, Julien Prunier, Vivek Srivastava, Don Stewart, Alex S. Torson, Emilie Tremblay, Adnan Uzunovic, Denys Yemshanov, Richard C. Hamelin. 2018 Biosurveillance of Forest Insects. Part II: Adoption of genomic tools by end user communities and barriers to integration. Journal of Pest Science 92 (1), 71-82. https://doi.org/10.1007/s10340-018-1001-1

 

Mbareche, Hamza,Veillette, Marc, Dubuis, Marie-Ève, Bakhiyi, Bouchra, Marchand, Geneviève, Zayed, Joseph, Lavoie, Jacques, Bilodeau, Guillaume J.,  and Duchaine, Caroline. 2018. Fungal bioaerosols in biomethanization facilities. Journal of the Air & Waste Management Association, 68 (11), P 1198-1210, https://doi.org/10.1080/10962247.2018.1492472

 

Johan Bengtsson-Palme, Rodney T Richardsson, Marco Meola, Christian Wurzbacher, Émilie D Tremblay, Kaisa Thorell, Kärt Kanger, Martin Eriksson, Guillaume J Bilodeau, Reed M Johnson, Martin Hartmann, Henrik Nilsson. 2018.Taxonomic identification from metagenomic and metabarcoding data using any genetic marker. Bioinformatics, Volume 34, Issue 23, 1 December 2018, Pages 4027–4033, https://doi.org/10.1093/bioinformatics/bty482

 

Feau, Nicolas, Stephanie Beauseigle, Marie-Josée Bergeron, Guillaume J Bilodeau, Inanc Birol, Sandra Cervantes-Arango, Braham Dhillon, Angela L Dale, Padmini Herath, Steven JM Jones, Josyanne Lamarche, Dario I Ojeda, Monique L Sakalidis, Greg Taylor, Clement KM Tsui, Adnan Uzunovic, Hesther Yueh, Philippe Tanguay, Richard C Hamelin. 2018. Genome-Enhanced Detection and Identification (GEDI) of plant pathogens. PeerJ 6:e4392 https://doi.org/10.7717/peerj.4392.

 

Simon Francis Shamoun, Danny Rioux, Brenda Callan, Delano James, Richard C. Hamelin, Guillaume J. Bilodeau, Marianne Elliott, C. André Lévesque, Elisa Becker, Dan McKenney, John Pedlar, Karen Bailey, S. C. Brière, Kurt Niquidet, and Eric Allen. 2018. An Overview of Canadian Research Activities on Diseases caused by Phytophthora ramorum: Results, Progress and Challenges .Plant Disease 102, (7) Pages 1218-1233 https://doi.org/10.1094/PDIS-11-17-1730-FE

 

Crandall, Sharifa G., Alamgir Rahman, Lina Maria Quesada-Ocampo, Frank N Martin, Guillaume Bilodeau, and Timothy D Miles. 2018. Advances in diagnostics of downy mildews: lessons learned from other oomycetes and future challenges. Plant Disease 102, 265-275. https://doi.org/10.1094/PDIS-09-17-1455-FE

 

2017

Si Ammour, M., Bilodeau, G. J., Tremblay, D. M., Van der Heyden, H., Yaseen, T., Varvaro, L., and Carisse, O. 2017. Development of real-time isothermal amplification assays for onsite detection of Phytophthora infestans in potato leaves. Plant Dis. In Press.

 

Miles, Timothy D., Martin, Frank N., Robideau, Gregg P., Bilodeau, Guillaume J., and Michael D. Coffey. 2017. Systematic development of Phytophthora species-specific mitochondrial diagnostic markers for economically important members of the genus. Plant Disease, In Press. http://dx.doi.org/10.1094/PDIS-09-16-1224-RE

 

Clime, Liviu, Li, Kebin, Geissler, Matthias,·Hoa , Xuyen D., Robideau, Gregg P., Bilodeau, Guillaume J. , and Teodor Veres. 2017. Separation and concentration of Phytophthora ramorum sporangia by inertial focusing in curving microfluidic flows. Microfluid Nanofluid 21:5 ; DOI 10.1007/s10404-016-1844-9

 

Gagnon, Marie-Claude, Feau, Nicolas, Dale,Angela Lynn, Dhillon, Braham, Hamelin, Richard. C, Brasier, Clive, Grünwald, Niklaus J., Brière, Stephan C., and Dr. Guillaume J. Bilodeau. 2017. Development and validation of polymorphic microsatellite loci for the NA2 lineage of Phytophthora ramorum from whole genome sequence data. Plant Disease, Volume 101, Number 5, Pages 666-673. http://dx.doi.org/10.1094/PDIS-11-16-1586-RE

2016

Feau, N., Taylor, G., Dale, A.L., Dhillon, B., Bilodeau, G. J. Birol, I., Jones, S. J. M. and Hamelin, R.C. (2016). Genome sequences of six Phytophthora species threatening forest ecosystems. Genomics Data 10: 85-88. http://dx.doi.org/10.1016/j.gdata.2016.09.013

 

Tambong, James Mr. Renlin Xu, Dr. Fouad Daayf, Mr. Stephan Briere, Guillaume Bilodeau, Mr. Raymond Tropiano, Allison Hartke, Lana M Reid, Morgan Cott, Tammy Cote, and Irina Agarkova. 2016. Genome analysis and development of a multiplex TaqMan real-time PCR for specific identification and detection of Clavibacter michiganensis subsp. Nebraskensis. Phytopathology. 106(12):1473-1485.

 

Gagnon, M-C. L.  T. A. J. van der Lee, P. J. M. Bonants, D. S. Smith, X. Li, C. A. Lévesque and G. J. Bilodeau,. 2016. Development of polymorphic microsatellite loci for potato wart from next-generation sequence data. Phytopathology, 106-6, P636-644.

 

Herath, P., S. Beauseigle, B. Dhillon1, D. I. Ojeda, G. Bilodeau, N. Isabel, M.-C. Gros-Louis, H. Kope, S. Zeglen, R. C. Hamelin, and Nicolas Feau. 2016. Anthropogenic signature in the incidence and distribution of an emerging pathogen of poplars. Biological Invasions 18:1147–1161.   DOI 10.1007/s10530-015-1051-8

2015

Gagnon, M-C. L.  Kawchuk, D., M.  Tremblay, O. Carisse, G. Danies, W .E. Fry, C. A. Lévesque and G. J. Bilodeau. 2015 Identification of the dominant genotypes of Phytophthora infestans in Canada using real-time PCR with ASO-PCR assays. Plant Disease, Vol. 100, No. 7: 1482-1491 (Accepted on December 31, 2015) http://dx.doi.org/10.1094/PDIS-07-15-0763-RE

Robideau, G. P., Foottit, R. G., Humble, L. M., Noseworthy, M. K., Wu, T. and Bilodeau, G. J. (2016), Real-time PCR identification of the ambrosia beetles, Trypodendron domesticum (L.) and Trypodendron lineatum (Olivier) (Coleoptera: Scolytidae). Journal of Applied Entomology 140:299-307. doi: 10.1111/jen.12254

 

Josyanne Lamarche, Amélie Potvin, Gervais Pelletier, Don Stewart, Nicolas Feau, Dario I. O. Alayon, Angela L. Dale, Aaron Coelho, Adnan Uzunovic, Guillaume J. Bilodeau, Stephan C. Brière, Richard C. Hamelin, Philippe Tanguay. 2015. Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR. PLoS ONE 10(8): e0134265. doi:10.1371/journal.pone.0134265

James Tabi Tambong, Renlin Xu, Zaky Adam, Morgan Cott,  Karin Rose, Lana Reid, Fouad Daayf, Stephan Brière, and Guilliame Bilodeau , 2015, Draft Genome Sequence of Clavibacter michiganensis subsp. nebraskensis  Strain DOAB 397 isolated from infected field corn plant in Manitoba, Canada., ASM Genome Announcements 3(4) · August 2015. DOI: 10.1128/genomeA.00768-15.

2014

Geissler, M., Li, K., Zhang, X., Clime, L., Robideau, G.P., Bilodeau, G.J., and T. Veres. 2014. Integrated air stream micromixer for performing bioanalytical assays on a plastic chip. Lab Chip, 14 (19), 3750-3761. 00, 1-12. DOI: 10.1039/c4lc00769g

 

Gagnon, M.-C., Bergeron, M.-J., Hamelin, R.C., Grunwald N.G. and G.J. Bilodeau. 2014. Real-time PCR assay to distinguish the four Phytophthora ramorum lineages using the cellulose binding elicitor lectin (CBEL) locus. Canadian Journal of Plant Pathology, 36, (3). 367-376.

 

Bilodeau, G. J., Martin, F. N., Coffey, M. D. and Blomquist, C. L. 2014. Development ofa multiplex assay for genus and species-specific detection of Phytophthora based on differences in mitochondrial gene order. Phytopathology 104 (7). 733-748. http://dx.doi.org/10.1094/PHYTO-09-13-0263-R

2012

Chen, W., Robleh Djama, Z., Coffey, M. D., Martin, F. N., Bilodeau, G.J., Radmer, L., Denton, G., and Lévesque, C. A., 2012. Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species. Phytopathology, 103:43-54.

 

Bilodeau G.J., S. T. Koike, P. Uribe, and F. N. Martin. 2012. Assay development for rapid detection and quantification of Verticillium dahliae in soil. Phytopathology, 102:331-343.

2011

Bilodeau G.J., 2011. Quantitative polymerase chain reaction for the detection of organisms in soil., CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources  6, No. 014:1-14.

2009

Bilodeau G.J., Pelletier, G., Pelletier, F., Lévesque, C. A., and R. C. Hamelin. 2009. Multiplex real-time polymerase chain reaction (PCR) for detection of Phytophthora ramorum, the causal agent of sudden oak death. Can. J. Plant. Pathol.  31(2): 195-210.

 

Niklaus J. Grünwald, Erica M. Goss, Kelly Ivors, Matteo Garbelotto, Frank N. Martin, Simone Prospero, Everett Hansen, Peter J.M. Bonants, Richard C. Hamelin, Gary Chastagner, Sabine Werres, David M. Rizzo, Gloria Abad, Paul Beales, Guillaume J. Bilodeau, et al. 2009 Standardizing the Nomenclature for Clonal Lineages of the Sudden Oak Death Pathogen, Phytophthora ramorum Phytopathology 99, Number 7: 792-795.

 

Martin, F.N., Coffey, M.D., Zeller, K., Hamelin, R., Tooley, P., Garbelotto, M., Hughes, K.J.D., Kubisiak, T., Bilodeau, G.J., Levy, L., Blomquist, C. and Berger, P. 2009 Evaluation of molecular markers for Phytophthora ramorum detection and identification; testing for specificity using a standardized library of isolates. Phytopathology 99: 390-403.

2008

Robideau, G.P., F.L., Caruso, P.V., Oudemans, P.S., McManus, M.A., Renaud, M.E., Auclair, G.J., Bilodeau, D., Yee, N.L., Desaulniers, J.W., DeVerna, and C.A., Lévesque. 2008. Detection of Cranberry Fruit Rot Fungi Using DNA Array Hybridization. Can. J. Plant. Pathol. 30 (2) : 226-240. (Erratum). Canadian Journal of Plant Pathology/Revue canadienne de phytopathologie, 30(4), p. 622.

2007

Bilodeau, G.J., C.A. Lévesque, A.W.A.M. de Cock, S.C. Brière, and R.C. Hamelin. 2007 Differentiation of european and north american genotypes of Phytophthora ramorum by real-time polymerase chain reaction primer extension. Can. J. Plant. Pathol. 29 (4): 408-420.

 

Bilodeau, G. J., C. A. Lévesque, A.W.A.M. de Cock, C. Duchaine, S. Brière, P. Uribe, F.N. Martin and R. C. Hamelin. 2007. Molecular Detection of Phytophthora ramorum by Real Time-PCR Using TaqMan, SYBR®Green and Molecular Beacons. Phytopathology 97 (5): 632-642.

Affiliations

Research Scientist, Canadian Food Inspection Agency (CFIA);

Adjunct Professor, Université Laval, Department of microbiology, biochemistry and bioinformatic;

Adjunct Research Professor, Carleton University, Department of biology;

Associate Editor : Canadian Journal of Plant Pathology

Editor: Archives Of Phytopathology And Plant Protection

Language

English
French

Other languages

Francais, Anglais