Dr. Muhammad Anzar

Image Muhammad Anzar
Research Scientist

Dr. Anzar is a Research Scientist (Cryobiology), Agriculture and Agri-Food Canada, Saskatoon Research Center; and Adjunct Professor, Dept. of Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada. He is focussing on cryopreservation of mammalian semen, oocytes, embryos and reproductive tissues for long term fertility preservation and conservation of Animal Genetic Resources of Canada.

Dr. Anzar teaches Reproductive Physiology to Biomedical Science, Biology and Animal Science students at University of Saskatchewan.


Current research and/or projects

I am mainly focussing on bio-secure cryopreservation of dairy, beef and bsion semen, oocytes and embryos, investigating the microbial causes of bulls' and cows' infertility, and developing fertility markers for breeding bulls.  

Research and/or project statements

  1. A genomic and metagenomic approach to quantifying the effect of climate change and environmental comtaminants on bison health ecology. A shared priority project "Genomic Adaptation and Resilience to Climate Change" among seven federal departments.
  2. Seminal microbiome and bull fertility
  3. Enhancement of mammalian gamete cryopreservation methods
  4. Bison Integrated genomics for recovery, resistance, and reconciliation (Big3R).
  5. Integrated Genomics for Sustainable Animal Agriculture and Environmental Stewardship

Professional activities / interests

  1. Research and Development 
  2. Teaching
  3. Graduate student supervision

Education and awards

D.V.M. College of Veterinary Medicine, Lahore, Pakistan

M.Sc. College of Veterinary Medicine, Lahore, Pakistan.

Ph.D. University of Minnesota, St. Paul, MN, USA

International experience and/or work

Lecturer, College of Veterinary Medicine, Lahore, Pakistan

Research Scientist, Pakistan Agricutural Research Council, Islamabad, Pakistan


Key publications

  1. Chohan, M.R., Munro, B.J., Cowan, V.E., Anzar, M., Blakley, B., McKinnon, J., Kastelic, J.P., Rivera-Acuña, F., Singh, J. (2021). Feeding yearling Angus bulls low-level ergot daily for 9 weeks decreased serum prolactin concentrations and had subtle effects on sperm end points. Theriogenology, [online] 161 187-199. http://dx.doi.org/10.1016/j.theriogenology.2020.11.025

    2021 - View publication details

  2. Yang, S.X., Adams, G.P., Zwiefelhofer, E.M., Rajapaksha, K., Anzar, M. (2021). Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility. Animal Reproduction Science, [online] 225 http://dx.doi.org/10.1016/j.anireprosci.2021.106691

    2021 - View publication details

  3. Anzar, M., Rajapaksha, K., Boswall, L. (2019). Egg yolk-free cryopreservation of bull semen. PLoS ONE, [online] 14(10), http://dx.doi.org/10.1371/journal.pone.0223977

    2019 - View publication details

  4. Beck, K., Singh, J., Dar, M.A., Anzar, M. (2018). Short-term culture of adult bovine ovarian tissues: Chorioallantoic membrane (CAM) vs. traditional in vitro culture systems. Reproductive Biology and Endocrinology, [online] 16(1), http://dx.doi.org/10.1186/s12958-018-0337-y

    2018 - View publication details

  5. Gupta, A., Singh, J., Dufort, I., Robert, C., Dias, F.C.F., Anzar, M. (2017). Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage. PLoS ONE, [online] 12(11), http://dx.doi.org/10.1371/journal.pone.0187268

    2017 - View publication details

  6. Arcarons, N., Morató, R., Vendrell, M., Yeste, M., López-Bejar, M., Rajapaksha, K., Anzar, M., Mogas, T. (2017). Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes. PLoS ONE, [online] 12(9), http://dx.doi.org/10.1371/journal.pone.0184714

    2017 - View publication details

  7. Cervantes, M.P., Palomino, J.M., Anzar, M., Mapletoft, R.J., Mastromonaco, G.F., Adams, G.P. (2017). In vitro-production of embryos using immature oocytes collected transvaginally from superstimulated wood bison (Bison bison athabascae). Theriogenology, [online] 92 103-110. http://dx.doi.org/10.1016/j.theriogenology.2017.01.017

    2017 - View publication details

  8. Cervantes, M.P., Palomino, J.M., Anzar, M., Mapletoft, R.J., Mastromonaco, G.F., Adams, G.P. (2017). In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes. Theriogenology, [online] 89 122-130. http://dx.doi.org/10.1016/j.theriogenology.2016.10.017

    2017 - View publication details

  9. Cervantes, M.P., Palomino, J.M., Anzar, M., Mapletoft, R.J., Adams, G.P. (2016). In vivo and in vitro maturation of oocytes collected from superstimulated wood bison (Bison bison athabascae) during the anovulatory and ovulatory seasons. Animal Reproduction Science, [online] 173 87-96. http://dx.doi.org/10.1016/j.anireprosci.2016.09.001

    2016 - View publication details

  10. Erickson, L., Kroetsch, T., Anzar, M. (2016). Relationship between sperm apoptosis and bull fertility: In vivo and in vitro studies. Reproduction, Fertility and Development, [online] 28(9), 1369-1375. http://dx.doi.org/10.1071/RD14417

    2016 - View publication details

  11. Gupta, A., Singh, J., Anzar, M. (2016). Effect of cryopreservation technique and season on the survival of in vitro produced cattle embryos. Animal Reproduction Science, [online] 164 162-168. http://dx.doi.org/10.1016/j.anireprosci.2015.11.026

    2016 - View publication details

  12. Kumar, A., Kroetsch, T., Blondin, P., Anzar, M. (2015). Fertility-associated metabolites in bull seminal plasma and blood serum: 1H nuclear magnetic resonance analysis. Molecular Reproduction and Development, [online] 82(2), 123-131. http://dx.doi.org/10.1002/mrd.22450

    2015 - View publication details

  13. Ahmad, M., Ahmad, N., Riaz, A., Anzar, M. (2015). Sperm survival kinetics in different types of bull semen: Progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation. Reproduction, Fertility and Development, [online] 27(5), 784-793. http://dx.doi.org/10.1071/RD13400

    2015 - View publication details

  14. Anzar, M., Grochulski, P., Bonnet, B. (2014). Synchrotron X-Ray diffraction to detect glass or ice formation in the vitrified bovine cumulus-oocyte complexes and morulae. PLoS ONE, [online] 9(12), http://dx.doi.org/10.1371/journal.pone.0114801

    2014 - View publication details

  15. Hussain, S.A., Lessard, C., Anzar, M. (2013). A strategy for improvement of postthaw quality of bison sperm. Theriogenology, [online] 79(1), 108-115. http://dx.doi.org/10.1016/j.theriogenology.2012.09.015

    2013 - View publication details

  16. Prentice-Biensch, J.R., Singh, J., Alfoteisy, B., Anzar, M. (2012). A simple and high-throughput method to assess maturation status of bovine oocytes: Comparison of anti-lamin A/C-DAPI with an aceto-orcein staining technique. Theriogenology, [online] 78(7), 1633-1638. http://dx.doi.org/10.1016/j.theriogenology.2012.07.013

    2012 - View publication details

  17. Prentice-Biensch, J.R., Singh, J., Mapletoft, R.J., Anzar, M. (2012). Vitrification of immature bovine cumulus-oocyte complexes: Effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development. Reproductive Biology and Endocrinology, [online] 10 http://dx.doi.org/10.1186/1477-7827-10-73

    2012 - View publication details

  18. Hussain, S.A., Lessard, C., Anzar, M. (2011). Quantification of damage at different stages of cryopreservation of endangered North American bison (Bison bison) semen and the effects of extender and freeze rate on post-thaw sperm quality. Animal Reproduction Science, [online] 129(3-4), 171-179. http://dx.doi.org/10.1016/j.anireprosci.2011.12.002

    2011 - View publication details

  19. Anzar, M., Kroetsch, T., Boswall, L. (2011). Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Animal Reproduction Science, [online] 126(1-2), 23-31. http://dx.doi.org/10.1016/j.anireprosci.2011.04.018

    2011 - View publication details

  20. Prentice, J.R., Singh, J., Dochi, O., Anzar, M. (2011). Factors affecting nuclear maturation, cleavage and embryo development of vitrified bovine cumulus-oocyte complexes. Theriogenology, [online] 75(4), 602-609. http://dx.doi.org/10.1016/j.theriogenology.2010.09.027

    2011 - View publication details

Research facility

107 Science Place
Saskatoon, SK S7N 0X2


  1. Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan (Adjunt Professor)



Other languages

Urdu, Punjabi