Dr. Linda J. Harris

Image Linda J. Harris
Research Scientist

Molecular pathologist studying Fusarium infection of cereals

Current research and/or projects

I am applying genomics and molecular biology to understand how the fungal pathogen Fusarium graminearum interacts with and infects cereal crops, and to characterize the biosynthesis and regulation of secondary metabolites, especially mycotoxins, of Fusarium species.

Recent publications:

Kebede AZ, Reid LM, Voloaca C, De Schiffart R, Wu J, Woldemariam T, Jindal KK, Zhu X, Bosnich W, Johnston A, Schneiderman D, Harris LJ. 2020. CO476 corn inbred line. Can. J. Plant Sci. Published online: July 15, 2020; DOI: 10.1139/CJPS-2020-0065

Brauer EK, Subramaniam R, Harris LJ. 2020. Regulation and dynamics of gene expression during the life cycle of Fusarium graminearum. Phytopathology, Published online: June 3, 2020; DOI: 10.1094/PHYTO-03-20-0080-IA

Eranthodi A, Schneiderman D, Harris LJ, Witte T, Sproule A, Hermans A, Overy D, Chatterton S, Liu J, Li T, Fundora DGP, Zhao W, Foroud NA. 2020. Enniatin production influences Fusarium avenaceum virulence on potato tubers, but not on durum wheat or peas. Pathogens 9(2), 75; DOI: 10.3390/pathogens9020075

Demissie Z, Witte T, Robinson K, Sproule A, Foote S, Johnston A, Harris LJ, Overy D, Loewen M. 2020. Transcriptomic and exometabolomic profiling reveals antagonistic and defensive modes of Clonostachys rosea action against Fusarium graminearum. MPMI 33:842-858; DOI: 10.1094/MPMI-11-19-0310-R

Xue AG, Chen Y, Seifert K, Guo W, Blackwell BA, Harris LJ, Overy, DP. 2020. Prevalence of Fusarium species causing head blight of spring wheat, barley and oat in Ontario during 2001-2017. Can. J. Plant Pathology 41: 392-402, DOI: 10.1080/07060661.2019.1582560

Research and/or project statements

Durable Fusarium Resistance

Mycotoxin biosynthesis and regulation in Fusarium graminearum

Characterization of new phytotoxic cyclic peptides of Fusarium graminearum

Genomic diversity and mycotoxigenic potential of Fusarium species in Canada

Professional activities / interests

Peer review panelist for EU Horizon 2020, 7th Framework Programme, Austrian Science Fund, USDA/ARS program and the Pathogen Biology & Genetics Research Area Committee of the U.S. Wheat & Barley Scab Initiative (USWBSI).

Conference organization - Chairperson of the National and local organizing committees of the 2009 6th Canadian Workshop on Fusarium Head Blight (CWFHB), member of organizing committees of the 12th European Fusarium Seminar in 2013,  2011 7th CWFHB, and 2012 MycoRed North American Workshop.

Member of Editorial Board of the journal Genes & Genomics

Education and awards

B.Sc. Biochemistry (Chemistry), Simon Fraser University

Ph.D. Genetics, University of British Columbia

International experience and/or work

NSERC Postdoctoral fellow, lab of Dr. Vicki Chandler, University of Oregon

Key publications

  1. Geiser DM, Al-Hatmi A, Aoki T, Arie T, et al. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex. Phytopathology. on-line 2020 Nov 17.

    2020 - View publication details

  2. Abstract Book of the 9th Canadian Workshop on Fusarium Head Blight & 4th Canadian Wheat Symposium, p. 49-50, https://www.cwfhb-cws.com/hotel-1/

    2019 - View publication details

  3. Nguyen HDT, Grunwald NJ, Abad ZG, Martin FN, Crouch J, Hambleton S, Dettman J, Liu M, Harris LJ, Castlebury L, Salagado-Salazar C, Cowger C, Ellouz O, Mollov D, Fall ML, Ling K, Griffifths J, Mahaffee W, Chen W, Sultata T, Yu Q, Carta LK, Skantar AM, Tambong J, Rogers E, Li S, Dumonceaux TJ, Widmer TL. The 2019 AAFC-USDA meeting on genotyping and monitoring of high-risk plant pathogens.

    2019 - View publication details

  4. Bahadoor, A., Brauer, E.K., Bosnich, W., Schneiderman, D., Johnston, A., Aubin, Y., Blackwell, B., Melanson, J.E., Harris, L.J. (2018). Gramillin A and B: Cyclic Lipopeptides Identified as the Nonribosomal Biosynthetic Products of Fusarium graminearum, 140(48), 16783-16791. http://dx.doi.org/10.1021/jacs.8b10017

    2018 - View publication details

  5. Harris, L.J., Johnston, A., Hermans, A., Sproule, A., Xue, A., Overy, D. Exploring the genetic diversity and mycotoxigenic potential of Canadian Fusarium poae isolates. 9th Can. Workshop on Fusarium Head Blight & 4th Can. Wheat Symp., https://www.cwfhb-cws.com/hotel-1/ Winnipeg, MB, Nov 19-22, 2018. P. 51. Poster.

    2018 - View publication details

  6. Plant Biology 2019 Abstract Book.

    2018 - View publication details

  7. Proceedings of the 14th European Fusarium Seminar, Tulln, Austria, Apr 8-11, 2018, P. 27, Invited talk. https://www.efs14.at/program.php

    2018 - View publication details

  8. Kebede, A.Z., Johnston, A., Schneiderman, D., Bosnich, W., Harris, L.J. (2018). Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes, 19(1), http://dx.doi.org/10.1186/s12864-018-4513-4

    2018 - View publication details

  9. Vachon, F., Overy, D.P., Hermans, A., Johnston, A., Sproule, A., Xue, A., Harris, L.J. Fusarium poae and emerging mycotoxins associated with fusarium head blight in Canada. Proceedings of 2017 Canadian Phytopathological Society – Eastern Ontario Regional Meeting, Ottawa, ON, Can. J. Plant Pathol. 40:3, 469.

    2017 - View publication details

  10. Subramaniam, G., Ouellet, T., Aboukhaddour, R., Blackwell, B., Fedak, G., Foster, A., Henriquez, M.A., Laroche, A., Larsen, J.; Miller, S., Overy, D., Rampitsch, C., Foroud, N., Schernthaner, J., Harris, L. 2017. Towards Durable Fusarium and Rust Resistance in Cereals. Wheat Science and Business Meeting, Saskatoon, November 8-9, 2017.

    2017 - View publication details

  11. Luo, K., DesRoches, C.L., Johnston, A., Harris, L.J., Zhao, H.Y., Ouellet, T. (2017). Multiple metabolic pathways for metabolism of l-tryptophan in Fusarium graminearum, 63(11), 921-927. http://dx.doi.org/10.1139/cjm-2017-0383

    2017 - View publication details

  12. DesRoches, C.-D., Johnston, A., Luo, K., Harris, L.J., Zhao, H.-Y., Ouellet, T. 2017. Effect of L-tryptophan, tryptamine and indole-3 acetaldehyde on gene expression in Fusarium graminearum. GEO accession# GSE100318. deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.

    2017 - View publication details

  13. Harris, L.J., Bahadoor, A., Brauer, E., Bosnich, W., Schneiderman, D., Aubin, Y., Melanson, J., Khan, I., Gleddie, S., Blackwell, B. Novel Fusarium graminearum cyclic peptides possess phytotoxic activity. 29th Fungal Genetics Conference, Pacific Grove, CA, Mar 14-19, 2017. Poster presentation.

    2017 - View publication details

  14. Kebede, A.Z., Harris, L.J.*, Johnston, A., Schneiderman, D., Bosnich, W., Woldemariam, T., Reid, L.M. Searching for candidate genes contributing to Fusarium graminearum resistance in a recombinant inbred population. 70th Northeast Corn Improvement Conference, Ottawa, ON, Feb 21-22, 2017. Oral presentation.

    2017 - View publication details

  15. Gunter, A.B., Hermans, A., Bosnich, W., Johnson, D.A., Harris, L.J., Gleddie, S. (2016). Protein engineering of Saccharomyces cerevisiae transporter Pdr5p identifies key residues that impact Fusarium mycotoxin export and resistance to inhibition, 5(6), 979-991. http://dx.doi.org/10.1002/mbo3.381

    2016 - View publication details

  16. Blackwell, B., Bahadoor, A., Bosnich, W., Schneiderman, D., Herweyer, D., Gräfenhan, T., Harris, L.J. Secondary metabolite profile of Canadian NX-2 Fusarium graminearum chemotypes. Proceedings of the 8th Can. Workshop on Fusarium Head Blight, Ottawa, ON Nov. 20-22, 2016. P. 66, Poster presentation.

    2016 - View publication details

  17. Harris, L.J.*, Bosnich, W., Johnston, A., Kwan, R., Schneiderman, D., Blackwell, B., Bahadoor, A., Gleddie, S. Examining Fusarium graminearum and Fusarium avenaceum interactions in durum wheat. Proceedings of the 8th Can. Workshop on Fusarium Head Blight, Ottawa, ON Nov. 20-22, 2016. P. 18, Invited talk.

    2016 - View publication details

  18. Kebede, A.Z., Johnston, A., Schneiderman, D., Bosnich, W., Reid, L.M., Harris, L.J. Combining QTL mapping with transcriptomics to identify candidate genes for Fusarium resistance in maize. Proceedings of the 8th Can. Workshop on Fusarium Head Blight, Ottawa, ON Nov. 20-22, 2016. P. 49, Poster presentation.

    2016 - View publication details

  19. Blackwell, B., Bahadoor, A., Bosnich, W., Schneiderman, D., Aubin, Y., Harris, L.J. NMR structural studies of Fusarium secondary metabolites. 29th Annual MOOT NMR Conference, Charlottetown, PEI, Sept 30 – Oct 2, 2016. Poster presentation.

    2016 - View publication details

  20. Demissie, Z., Harris, L., Ouellet, T., Subramaniam, G., Schernthaner, H., Singh, J., Gleddie, S., Vrinten, P. and Loewen, M. 2016. Linking ABC transporters to Fusarium graminearum resistance and susceptibility in wheat: a CWA focus group. Third Annual Science Meeting of the Canadian Wheat Alliance, Saskatoon, June 27-28, 2016.

    2016 - View publication details