Dr. Jian Wang, Ph.D

Research Scientist, Head of Research and Development Unit

Lead and plan research on analysis of chemical contaminants including pesticides and veterinary drugs in food, and food authenticity using emerging mass spectrometric technologies.

Develop and implement non-target data acquisition for target analysis (nDATA) using high resolution mass spectrometry for food safety.

Canadian Food Inspection Agency
Calgary, Alberta

Current research and/or projects

  1. UHPLC/ESI Q-Orbitrap and UHPLC/ESI QTRAP 6500+ plus SelexION DMS applications for target and non-target analyses of
    1. Pesticide residues in fruits, vegetables, infant food, tea, grains, pulses, juices etc.
    2. Veterinary drug residues in milk, eggs, and honey.
  2. HRMS-based metabolomics for food authenticity, especially juices.
  3. Estimation of measurement uncertainty (statistics and SAS programing).

Research and/or project statements

Intelligent foresight to improve science and identify analytical gaps that are between current science capabilities and those required to address future threats for chemical contaminant analysis related to food safety and quality mandate, and implementation of emerging technologies to safeguard Canadian food supply.

Professional activities / interests

  1. Develop nDATA workflow for analysis of chemical contaminants in food.
  2. Expand compound databases including pesticides, veterinary drugs and mycotoxins, a total over 1500 compounds, for nDATA in food safety applications.
  3. Promote nDATA for improving minoring program efficiency and risk intelligence for analysis of chemical contaminant in food.

Education and awards

EDUCATION

  1. Ph. D., Food Chemistry, Food Science and Technology, University of Alberta, Edmonton, Alberta, Canada, 2000.
  2. M. Sc., Cereal Chemistry, Grain Science, Henan University of Technology, Zhenzhou, Henan, P.R. China, 1989.
  3. B. Sc., Grain Science, Henan University of Technology, Zhengzhou, Henan, P.R. China, 1986.

AWARDS

  1. Fellow of AOAC INTERNATIONAL, 2018.
  2. CFIA President's National Award for Innovation and Best Practices, 2017.
  3. AOAC INTERNATIONAL Expert Review Panel (ERP) of the Year, 2015.
  4. CFIA Science Branch Recognition Award for leading method development on melamine investigation, 2007.

International experience and/or work

  1. Member of the Canadian delegation on the Codex Committee on Pesticide Residues (CCPR).
  2. Canadian coordinator on Codex Committee on Pesticide Residues Working Group on Guidelines of method performance for analysis of pesticides and veterinary drug residues in food.
  3. Canadian coordinator on Codex Committee on Pesticide Residues Working Group on Guidance for the monitoring of the Purity and Stability of Certified Reference Materials (CRMs).
  4. Member of North American Chemical Residue Workshop Program Committee.
  5. Member of AOAC INTERNATIONAL Membership Executive Committee.
  6. Research collaborations with US FDA, ThermoFisher Scientific and universities.
  7. Member of AOAC INTERNATIONAL Technical Programming Council.
  8. Member of the Joint FAO/WHO Expert Committee on Food Additives and Contaminants (JECFA).
  9. Member of Codex Committee on Residues of Veterinary Drugs in Foods (CCRVDF) Working Group for “CAC-GL 71-2009, Appendix C-Performance Characteristics for Multi-Residue Methods (MRMs) for Veterinary Drugs”.
  10. Executive Committee, Scientific Program Organizing Committee and Local Organizing Committee member for 2nd (2011) and 3rd (2015) SaskVal, i.e. the Saskatoon International Workshop on Validation and Regulatory Analysis.

Key publications

  1. Wang, J.; Chow, W. The Study of Ultra-high Performance Liquid Chromatography Electrospray Ionization Q-Orbitrap Mass Spectrometry and Various Extraction Methods for Fingerprinting and Identification of Markers from Apple Juices and Grape Juices. ACS Food Sci. Technol. doi.org/10.1021/acsfoodscitech.2c00153.
  2. Chow, W.; Leung, D.; Wang, J. A Complementary, Quantitative and Confirmatory Method to UHPLC/ESI-HRMS, based on UPHLC-MS/MS that can Analyze up to 416 Pesticides in Fruits and Vegetables. J. AOAC Int. 1-20. doi: 10.1093/jaoacint/qsab116.
  3. Wang, J.; Chow, W.; Wong, J. W.; Chang, J. Applications of nDATA for screening, quantitation and identification of pesticide residues in fruits and vegetables using UHPLC/ESI Q-Orbitrap all ion fragmentation and data independent acquisition. J. Mass Spectrom. 2021, 56, e4783.
  4. Wong, J. W.; Wang, J.; Chang, J.; Chow, W.; Carlson, R.; et al. A Multilaboratory Collaborative Study of an non-Target Data Acquisition for Target Analysis (nDATA) Workflow using Liquid Chromatography-High Resolution Accurate Mass Spectrometry for Pesticide Screening in Fruits and Vegetables. Dr. Jon W Wong and Dr Jian Wang shared equal contribution and are the corresponding authors. J.  Agric. Food Chem. 2021, 69, 13200-13216.
  5. Wang, J.; Leung, D.; Chow, W.; Wong, JW.; Chang, J. UHPLC/ESI Q-Orbitrap Quantitation of 655 Pesticide Residues in Fruits and Vegetables – a Companion to an nDATA Working Flow. J. AOAC Int. 2020, 103 (6), 1547-1559.
  6. Wang, J.; Chow, W.; Wong, JW.; Leung, D.; Chang, J.; Li, M. Non-target data acquisition for target analysis (nDATA) of 845 pesticide residues in fruits and vegetables using UHPLC/ESI Q-Orbitrap. Anal. Bioanal. Chem. 2019, 7, 1421-1431. doi:10.1007/s00216-019-01581-z
  7. Wong, J.W.; Wang, J.; Chow, W.; Carlson, R.; Jia, Z.; Zhang, K.; Hayward, D.G.; Chang, J. Perspectives on liquid chromatography–high-resolution mass spectrometry for pesticide screening in foods. J. Agri. Food Chem. 2018, 66: 9573-9581. doi:10.1021/acs.jafc.8b03468.
  8. Wang, J.; Leung, D.; Chow, W.; Chang, J.; Wong, J.W. Target screening of 105 veterinary drug residues in milk using UHPLC/ESI Q-Orbitrap multiplexing data independent acquisition. Anal. Bioanal. Chem. 2018, 410 (22):5373-5389. doi:10.1007/s00216-017-0847-z.
  9. Wang, J.; Chow, W.; Chang, J.; Wong, J. W. Development and Validation of a Qualitative Method for Target Screening of 448 Pesticide Residues in Fruits and Vegetables Using UHPLC/ESI Q-Orbitrap Based on Data-Independent Acquisition and Compound Database. J. Agric. Food Chem. 2017, 65: 473-493.
  10. Wang, J.; Cheung, W.; Chow, W., UHPLC/ESI-MS/MS determination of 187 pesticides in wine. J. AOAC Int. 2016, 99, 539-557.
  11. Wang, J.; Leung, D.; Chow, W.; Chang, J.; Wong, J. W., Development and validation of a multiclass method for analysis of veterinary drug residues in milk using ultrahigh performance liquid chromatography electrospray ionization quadrupole Orbitrap mass spectrometry. J. Agric. Food Chem. 2015, 63, 9175-9187.
  12. Yang, P.; Chang, J.; Wong, J.; Zhang, K.; Krynitsky, A.; Bromirski, M.; Wang, J. Effect of sample dilution on matrix effects in pesticide analysis of several matrices by liquid chromatography- high resolution mass spectrometry. J. Agri. Food Chem. 2015, 63, 5169-5177.
  13. Wang, J.; Cheung, W.; Chang, J.; Wong, J. W., Ultrahigh-performance liquid chromatography electrospray ionization Q-Orbitrap mass spectrometry for the analysis of 451 pesticide residues in fruits and vegetables: method development and validation. J. Agri. Food Chem. 2014, 62, 10375-10391.
  14. Wang, J.; Cheung, W.; Leung, D., Determination of pesticide residue transfer rates (percent) from dried tea leaves to brewed tea. J. Agri. Food Chem. 2014, 62, 966-983.
  15. Wang, J.; Cheung, W.; Chow, W., UHPLC/ESI-MS/MS analysis of 151 pesticides in soybeans and pulses. J. AOAC Int. 2013, 96, 1114-1133.
  16. Wang, J.; Chow, W.; Leung, D.; Chang, J., Application of ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry for determination of 166 pesticides in fruits and vegetables. J. Agric. Food Chem. 2012, 60, 12088-12104.
  17. Wang, J.; Leung, D., The challenges of developing a generic extraction procedure to analyze multi-class veterinary drug residues in milk and honey using ultra-high pressure liquid chromatography quadrupole time-of-flight mass spectrometry. Drug. Test Anal. 2012, 4, (SUPPL.1), 103-111.
  18. Wang, J.; Chow, W.; Leung, D. Applications of LC/ESI-MS/MS and UHPLC QqTOF MS for the Determination of 141 Pesticides in Tea. J. AOAC Int. 2011, 94(6), 1-30.
  19. Wang, J.; Chow, W.; Cheung, W. Application of a tandem mass spectrometer and Core-shell particle column for the determination of 151 pesticides in grains. J. Agric. Food Chem. 2011, 59, 8589-8608.
  20. Wang, J.; Chow, W.; Leung D. Applications of LC/ESI-MS/MS and UHPLC QqTOF MS for the determination of 148 pesticides in fruits and vegetables Anal. Bioanal. Chem. 2010, 396(4), 1513-1538.
  21. Wang, J.; Leung, D.; Chow, W. Applications of LC/ESI-MS/MS and UHPLC QqTOF MS for the determination of 148 pesticides in berries. J. Agric. Food Chem. 2010, 58(10), 5904-5925.
  22. Dickson, L.C.; Wang J. Analysis of residues of macrolide antibiotics in animal-derived foods. Food Eng. Ingredients. 2010, 35 (SPEC. ISSUE), 18-21.
  23. Wang, J.; Leung, D. Determination of 142 pesticides in fruit- and vegetable-based infant foods using liquid chromatography electrospray ionization tandem mass spectrometry and estimation of measurement uncertainty. J. AOAC Int. 2009, 92, 279-301.
  24. Wang, J.; Leung, D. Applications of ultra-performance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry on analysis of 138 pesticides in fruit- and vegetable-based infant foods. J. Agric. Food Chem. 2009, 57, 2162-2173.
  25. Wang, J.; Leung, D. Determination of spiramycin and neospiramycin antibiotic residues in raw milk using LC/ESI-MS/MS and solid-phase extraction. J. Sep. Sci. 2009, 32, 681-688.
  26. Wang, J. Analysis of macrolide Antibiotics using Liquid chromatography mass spectrometry, in food, biological and environmental matrices. Mass Spectrom. Reviews. 2009, 28, 50-92.
  27. Wang, J.; Leung, D. Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2007, 21, 3213-3222.
  28. Wang, J.; Wotherspoon, D. Determination of pesticides in apples using liquid chromatography electrospray ionization tandem mass spectrometry and measurement uncertainty. J. AOAC Int. 2007, 90, 55 -567.
  29. Wang, J.; Leung, D.; Lenz, S, P. Determination of five macrolide antibiotic residues in raw milk using liquid chromatography/electrospray ionization tandem mass spectrometry. J. Agric. Food Chem. 2006, 54, 2873-2880.
  30. Wang, J.; Cheung, W. Determination of pesticides in soy-based infant formula using liquid chromatography with electrospray ionization tandem mass spectrometry. J. AOAC Int. 2006, 89, 214-224.
  31. Wang, J.; Leung, D.; Butterworth, F. Determination of five macrolide antibiotic residues in eggs using liquid chromatography/electrospray ionization tandem mass spectrometry. J. Agric. Food Chem. 2005, 53, 1857-1865.
  32. Wang, J.; Cheung, W.; Grant, D. Determination of pesticides in apple-based infant foods using liquid chromatography electrospray ionization tandem mass spectrometry. J. Agric. Food Chem. 2005, 53, 528-537.
  33. Wang, J. Determination of six penicillins in honey: Confirmatory method using liquid chromatography electrospray ionization tandem mass spectrometry. J. AOAC Int. 2004, 87, 45-55.
  34. Wang, J. Determination of five macrolide antibiotic residues in honey by LC/ESI-MS and LC/ESI-MS/MS. J. Agric. Food Chem. 2004, 52, 171-181.
  35. Kay, C. D.; Mazza, G.; Holub, B. J.; Wang, J. Anthocyanin metabolites in human urine and serum. Brit. J. Nutr. 2004, 91, 933–942.
  36. Wang, J.; Mazza, G. Quantification of nitrite in the presence of anthocyanins using Griess and GC/MS Assays. J. Food Biochem. 2002, 26, 339-354.
  37. Wang, J.; Mazza, G. Inhibitory effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor-a in LPS/IFN-g activated RAW 264.7 macrophages. J. Agric. Food Chem. 2002, 50, 4183-4189.
  38. Wang, J.; Mazza, G. Inhibitory effects of anthocyanins and other phenolic compounds on nitric oxide production in LPS/IFN-g activated RAW 264.7 macrophages. J. Agric. Food Chem. 2002, 50, 850-857.
  39. Wang, J.; Sporns, P. MALDI-TOF MS analysis of isoflavones in soy products. J. Agric. Food Chem. 2000, 48, 5887-5892.
  40. Wang, J.; Kalt, W.; Sporns, P. Comparison between HPLC and MALDI-TOF MS analysis of anthocyanins in highbush blueberries. J. Agric. Food Chem. 2000, 48, 3330-3335.
  41. Wang, J.; Sporns, P. MALDI-TOF MS quantification of coccidiostats in poultry feeds. J. Agric. Food Chem. 2000, 48, 2807-2811.
  42. Wang, J.; Sporns, P. MALDI-TOF MS analysis of food flavonol glycosides. J. Agric. Food Chem. 2000, 48, 1657-1662.
  43. Wang, J.; Jiang, G. S.; Vasanthan, T.; Sporns, P. MALDI-MS characterization of maltooligo/polysaccharides from debranched starch amylopectin of corn and barley. Starch. 1999, 51, 243-248.
  44. Wang, J.; Sporns, P. Analysis of anthocyanins in red wine and fruit juice using MALDI-MS.  J. Agric. Food Chem. 1999, 47, 2009-2015.
  45. Wang, J.; Sporns, P.; Nicholas H. L. Analysis of food oligosaccharides using MALDI-MS: quantification of fructooligosaccharides. J. Agric. Food Chem. 1999, 47, 1549-1557.
  46. Sporns, P.; Wang, J. Exploring new frontier in food analysis using MALDI-MS. Food Res. Int. 1998, 31, 181-189.

BOOKS:

  1. Wang, J.; MacNeil, J.; Kay, J. Editors. Chemical Analysis of Antibiotic Residues in Food. Publisher: John Wiley & Sons, Inc. 2012.
  2. Kay, J.;  MacNeil, J.; Wang, J. Editors. Chemical Analysis of Non-antimicrobial Veterinary Drug Residues in Food. Publisher: John Wiley & Sons, Inc. 2016.

BOOK CHAPTERS:

  1. Wang, J.; Turnipseed, S. Chapter 6. Chemical analysis: quantitative and confirmatory methods. In Chemical Analysis of Antibiotic Residues in Food. Publisher: John Wiley & Sons, Inc. Editors: Wang, J.; MacNeil, J.; Kay, J. 2012.
  2. Wang, J.; Cannavan, A.; Dickson, L.; Fedeniuk, R. Chapter 9. Measurement uncertainty. In Chemical Analysis of Antibiotic Residues in Food. Publisher: John Wiley & Sons, Inc. Editors: Wang, J.; MacNeil, J.; Kay, J. 2012.
  3. Wang, J. Chapter 38. Analysis of antibiotics in milk and its products. In Handbook of Dairy Foods Analysis. Publisher: CRC Press. Editors: Nollet, L.M.L.; Toldrá, F. 2010.
  4. Wong, J. W.; Wang, J.; Zhang, K.; Hayward, D. G.; Yang, P.; Wittenberg, J. B. Pesticides: an update on mass spectrometry approaches.  In:  Encyclopedia of Food Chemistry, Melton, L., Shahidi, F., Varelis, P. (Eds), Elsevier, ISBN: 9780128140260. 2019.
  5. Wong, J. W.; Zhang, K.; Hayward, D.G.; Krynitsky, A. J.; Wittenberg, J.; Wang, J.; Yang, P.  Determination of pesticides in food of vegetal origin.  In: Analysis of Pesticides in Food and Environmental Samples, Second Edition.  J. Tadeo, Ed., CRC Press, Taylor & Francis Group, Boca Rotan, FL, USA, ISBN 9781138486034. 2019.

Contact

Research facility

Calgary Laboratory
3650 36th St. N.W.
Calgary, AB T2L 2L1
Canada

Expertise

Food›Chemistry›Contaminants
Food›Chemistry›Drug residues
Food›Chemistry›Pesticide residues

Affiliations

Member of AOAC International

Language

English