Dr. Christof Rampitsch

Image Christof Rampitsch
Research Scientist

Research Scientist in Proteomics and Biological Mass Spectrometry.

Current research and/or projects

My programme uses proteomics, peptidomics, bioinformatics and biological mass spectrometry to investigate plant-pathogen interactions.  Research is mainly focused on Puccinia triticina (wheat leaf rust), with emphasis on effector proteins and peptides, and on Fusarium graminearum, with emphasis on mycotoxin biosynthesis regulation.  

Our lab collaborates extensively with other researchers who use proteomics but lack the expertize, equipment and software to address complex biological questions.  These projects currently involve complex analyses using stable-isotope-labelled tags, label-free quantification, and analyzing post-translational modifications (phosphorylation and redox modifications).

Research and/or project statements

Projects:

  • Stomata signalling pathways for increasing yield potential in wheat
  • Mitigating Emerging Threats to Wheat in Canada
  • Integrated genomics, plant pathology and breeding research for improvement of Fusarium Head Blight resistance in Canadian cereals
  • Developing “prairie cold tolerance” for early-season germination and establishment in prairie crops

Professional activities / interests

Host-pathogen interactions

Proteomics

Peptidomics

Biological Mass Spectrometry

Education and awards

Ph.D.  Plant Science, University of British Columbia, Vancouver BC, Canada.

M.Sc., Biotechnology, University of the Witwatersrand, Johannesburg, South Africa.

B.Sc. (Hons) Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.

International experience and/or work

  • Hardware Training: LTQ mass spectrometer (Thermo Training Centre, Palm Beach FL)
  • Hardware Training: nano HPLC (Dionex Training Centre, Chicago IL)
  • Software Training: Mascot (MatrixScience Course, Savannah GA) 
  • Sabbatical Leave, University of Zürich/ETH Functional Genomics Centre Zürich CH.
  • Hardware Training: Q Exactive mass spectrometer (Thermo, on-site)
  • Software training, MaxQuant, Berlin (Summer School)

Key publications

  1. Eranthodi A, Subramaniam R, Rampitsch C, Ouellet T, Gonzàlez-Peña Fundora D, Vivar M, Zhao W, Goyal RK, Schultz EA, Foroud NA. Fusarium graminearum mutants towards identification of pathogenesis-associated molecular patterns. 2nd Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 30, 2016. Award Winning Presentation

    2016 - View publication details

  2. Foroud, N.A., Eranthodi, A., Goyal, R.K., Gonzàlez-Peña Fundora, D., Zhao, W., Vivar, M., Ouellet. T., Subramaniam, R., Rampitsch, C. 2016. Characterization of a secreted Fusarium graminearum elongation factor – an elicitor of host defence? Proceedings of the 8th Canadian Workshop on Fusarium Head Blight, Ottawa, ON, November 20-22 2016. P 19. Invited Oral Presentation.

    2016 - View publication details

  3. Eranthodi A, Subramaniam R, Rampitsch C, Ouellet T, Vivar M, Gonzàlez-Peña Fundora D, Zhao W, Goyal RK, Schultz EA, Foroud NA. Characterization of three Fusarium graminearum overexpression mutants and their ability to elicit host defence response in cereals. 8th Canadian Workshop of Fusarium Head Blight, Ottawa ON, CANADA, November 20-22, 2016. p.61

    2016 - View publication details

  4. Rampitsch, C., Bacala, R., Tekauz, A., McCallum, B.D. (2016). Inhibition of Fusarium graminearum and other Fusarium species by Cochlioblolus sativus in culture and on barley plants. Canadian Journal of Plant Pathology, [online] 38(4), 422-429. http://dx.doi.org/10.1080/07060661.2016.1243583

    2016 - View publication details

  5. Fan, T., Bykova, N.V., Rampitsch, C., Xing, T. (2016). Identification and characterization of a serine protease from wheat leaves. European Journal of Plant Pathology, [online] 146(2), 293-304. http://dx.doi.org/10.1007/s10658-016-0914-x

    2016 - View publication details

  6. Sivagnanam, K., Komatsu, E., Patrick, S., Rampitsch, C., Perreault, H., Gräfenhan, T. (2016). Rapid screening of ergot alkaloids in sclerotia by maldi-tof mass spectrometry, 99(4), 895-898. http://dx.doi.org/10.5740/jaoacint.16-0117

    2016 - View publication details

  7. McCallum, B.D., Hiebert, C.W., Cloutier, S., Bakkeren, G., Rosa, S.B., Humphreys, D.G., Marais, G.F., McCartney, C.A., Panwar, V., Rampitsch, C., Saville, B.J., Wang, X. (2016). A review of wheat leaf rust research and the development of resistant cultivars in Canada. Canadian Journal of Plant Pathology, [online] 38(1), 1-18. http://dx.doi.org/10.1080/07060661.2016.1145598

    2016 - View publication details

  8. Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Towards the identification and characterization of pathogen-associated molecular patterns from the Fusarium graminearum secretome.", 1st Lethbridge Research Centre Graduate Student Symposium, Lethbridge, AB, Canada, November 26, 2015, Award Winning: 2nd Place for Oral Presentation.

    2015 - View publication details

  9. Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Fusarium graminearum mutant screening towards identification of pathogen-associated molecular patterns in the Fusarium head blight-wheat interaction.", 36th Annual Meeting of the Plant Pathology Society of Alberta, Lethbridge, AB, Canada, November 16-18, 2015, Oral Presentation.

    2015 - View publication details

  10. Eranthodi, A., Subramaniam, R., Rampitsch, C., Ouellet, T., Schultz, E.A., and Foroud, N.A. (2015). "Generation of Fusarium graminearum mutants to screen candidate pathogen-associated molecular patterns.", National Fusarium Head Blight Forum, St. Louis, MO, USA, December 6-8, 2015. (Poster)

    2015 - View publication details

  11. Hu, J., Rampitsch, C., Bykova, N.V. (2015). Advances in plant proteomics toward improvement of crop productivity and stress resistance, 6(APR), http://dx.doi.org/10.3389/fpls.2015.00209

    2015 - View publication details

  12. Rampitsch, C., Günel, A., Beimcik, E., Mauthe, W. (2015). Proteome of monoclonal antibody-purified haustoria from Puccinia triticina Race-1, 15(7), 1307-1315. http://dx.doi.org/10.1002/pmic.201400241

    2015 - View publication details

  13. Day, J., Gietz, D.D., Rampitsch, C. (2015). Proteome changes induced by Pyrenophora tritici-repentis ToxA in both insensitive and sensitive wheat indicate senescence-like signaling, 13(1), http://dx.doi.org/10.1186/s12953-014-0060-3

    2015 - View publication details

  14. Rampitsch, C., Beimcik, E., Bakkeren, G., Linning, R., Joly, D.L., and McCallum, B.D. (2014). "Quantitative proteomics with custom database searching to reveal differences in the haustorial proteomes of three races of wheat leaf rust, Puccinia triticina.", 12th European Conference on Fungal Genetics, Sevilla, Spain, March 2014.

    2014 - View publication details

  15. Bakkeren, G., Fellers, J.P., Panwar, V., Joly, D.L., Szabo, L.J., Kolmer, J.A., Hulbert, S., Wang, M., Yin, C., Chen, X., Rampitsch, C., McCallum, B.D., Wang, X., Goldberg, J., Young, S., Hamelin, R.C., Saville, B.J., and Cuomo, C.A. (2014). "Comparative genomics of rust fungi drives research and innovations for crop protection.", 16th conference of the International Society for Molecular Plant-Microbe Interactions, Rhodes, Greece, July 6-10, 2014.

    2014 - View publication details

  16. Bakkeren, G., Fellers, J.P., Panwar, V., Joly, D.L., Szabo, L.J., Kolmer, J.A., Hulbert, S., Wang, M., Yin, C., Chen, X., Rampitsch, C., McCallum, B.D., Wang, X., Goldberg, J., Young, S., Hamelin, R.C., Saville, B.J., and Cuomo, C.A. (2014). "Comparative genomics of rust fungi drives research and innovations.", XXII Plant & Animal Genome Conference, Town & Country Convention Center, San Diego, CA, USA, January 10-15, 2014.

    2014 - View publication details

  17. Rampitsch, C. et al. (2014). "Quantitative proteomics with custom database searching to reveal differences in the haustorial proteomes of three races of wheat leaf rust, Puccinia triticina.", 12th European Conference on Fungal Genetics, Sevilla, Spain. (Poster)

    2014 - View publication details

  18. Bykova, N.V., Rampitsch, C., and others (2014). "Proteomic approaches to revealing mechanisms of physiological transition from seed dormancy to high germination potential in wheat.", Plant Biology 2014, Portland, Oregon, USA, July 12-16, 2014. (Poster)

    2014 - View publication details

  19. Rampitsch, C., Bakkeren, G., and McCallum, B.D. (2014). "Custom database searching to reveal differences in the haustoria proteomes of three races of wheat leaf rust, Puccinia triticina.", 1st Meeting of the International Plant Proteomics Organization, Hamburg, Germany, August 31- September 4, 2014. (Presentation)

    2014 - View publication details

  20. Ponts, N., Djuric-Ciganovic, S., and Rampitsch, C. (2014). "Analysis of Post-translational Modifications of Histones in Fusarium graminearum.", Canadian Workshop on Tandem Mass Spectrometry, Lake Louise, Canada. (Poster)

    2014 - View publication details

Research facility

Route 100, Unit 100-101
Morden, MB R6M 1Y5
Canada

Affiliations

Editorial Board, Proteomics.

Associate Editor (Microbial Genetics, Genomics and Proteomics), BMC Microbiology.

Language

English

Other languages

Deutsch