Dr. Christof Rampitsch

Image Christof Rampitsch
Research Scientist

Research Scientist in Proteomics and Biological Mass Spectrometry.

Current research and/or projects

My programme uses proteomics, peptidomics, bioinformatics and biological mass spectrometry to investigate plant-pathogen interactions.  Research is mainly focused on Puccinia triticina (wheat leaf rust), with emphasis on effector proteins and peptides, and on Fusarium graminearum, with emphasis on mycotoxin biosynthesis regulation.  

Our lab collaborates extensively with other researchers who use proteomics but lack the expertize, equipment and software to address complex biological questions.  These projects currently involve complex analyses using stable-isotope-labelled tags, label-free quantification, and analyzing post-translational modifications (phosphorylation and redox modifications).

Research and/or project statements

Projects:

  • Stomata signalling pathways for increasing yield potential in wheat
  • Mitigating Emerging Threats to Wheat in Canada
  • Integrated genomics, plant pathology and breeding research for improvement of Fusarium Head Blight resistance in Canadian cereals
  • Developing “prairie cold tolerance” for early-season germination and establishment in prairie crops

Professional activities / interests

Host-pathogen interactions

Proteomics

Peptidomics

Biological Mass Spectrometry

Education and awards

Ph.D.  Plant Science, University of British Columbia, Vancouver BC, Canada.

M.Sc., Biotechnology, University of the Witwatersrand, Johannesburg, South Africa.

B.Sc. (Hons) Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.

International experience and/or work

  • Hardware Training: LTQ mass spectrometer (Thermo Training Centre, Palm Beach FL)
  • Hardware Training: nano HPLC (Dionex Training Centre, Chicago IL)
  • Software Training: Mascot (MatrixScience Course, Savannah GA) 
  • Sabbatical Leave, University of Zürich/ETH Functional Genomics Centre Zürich CH.
  • Hardware Training: Q Exactive mass spectrometer (Thermo, on-site)
  • Software training, MaxQuant, Berlin (Summer School)

Key publications

  1. Bykova NV, Rampitsch C, McCallum B, Bakkeren G, Wang X, Huang M, Fernando U, Radovanovic N, Rampitsch M, Linning R (2021) Profiling the total and nuclear proteomes of host-pathogen interactions of the wheat Thatcher near-isogenic line for Lr2a resistance gene with virulent and avirulent races of Puccinia triticina. 4th International Plant Proteomics Organization (INPPO) Conference. Virtual Conference hosted from Winnipeg, Canada, March 9-11, 2021. Poster http://www.inppo2020.org/

    2021 - View publication details

  2. Rampitsch M, Huang M, Zhen Y, Radovanovic N, Xu W, Rampitsch C, Bykova NV (2019). Profiling of histone acetylation and methylation marks associated with embryo and aleurone tissue-specific epigenetic regulation of seed dormancy in wheat. ASMS Conference on Mass Spectrometry and Allied Topics. Atlanta, Georgia, June 2 - 6, 2019. Poster presentation, Abstract ID number: 299846.

    2019 - View publication details

  3. Eranthodi A, Subramaniam R, Rampitsch C, Ouellet T, González-Peña Fundora D, Schultz EA, Foroud NA. 2018. Characterization of three cysteine-rich proteins from the secretome of Fusarium graminearum. 9th Canadian Workshop of Fusarium Head Blight and the 4th Canadian Wheat Symposium Joint Meeting, Winnipeg MB, CANADA. Nov 19-22, 2018. (Oral Presentation)

    2018 - View publication details

  4. González-Peña Fundora D, Eranthodi A, Subramaniam R, Rampitsch C, Thakor N, Foroud NA. 2018. Overexpression of the Fusarium graminearum mitogen-activated protein kinase, Mgv1, does not affect vegetative growth or virulence. 4th Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 15, 2018. Award Winning Presentation.

    2018 - View publication details

  5. Eranthodi A, Subramaniam R, Ouellet T, Rampitsch C, Shostak K, González-Peña Fundora D, Schultz EA, Foroud NA. 2018. The role of three secreted cysteine-rich proteins of Fusarium graminearum fungal growth and host infection. 4th Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 15, 2018.

    2018 - View publication details

  6. Bykova NV, Rampitsch M, Rampitsch C, Huang M (2018) Histone posttranslational modification landscape related to epigenetic regulation of seed dormancy in wheat. 3rd International Plant Proteomics Organization (INPPO) World Congress. Padova, Italy, September 9-12, 2018. Poster presentation

    2018 - View publication details

  7. Rampitsch C, Bykova NV, Wang X, Huang M, Djuric-Ciganovic S (2018) Candidate Effector Proteome of the apoplastic space of wheat leaves infected with leaf rust (Puccinia triticina). 3rd International Plant Proteomics Organization (INPPO) World Congress. Padova, Italy, September 9-12, 2018. Oral presentation

    2018 - View publication details

  8. González-Peña Fundora D, Eranthodi A, Goyal RK, Subramaniam R, Rampitsch C, Thakor N, Foroud NA. 2018. Effects of over-expressing the Mgv1 mitogen-activated protein kinase in Fusarium graminearum. 2018 Canadian Phytopathological Society Annual Meeting. Québec QC, CANADA, June 18-22, 2018. Published Abstract in the Canadian Journal of Plant Pathology. DOI:10.1080/07060661.2019.1519163

    2018 - View publication details

  9. Huang M, Fernando U, Djuric-Ciganovic S, Wang X, Bakkeren G, Linning R, Bykova NV, Rampitsch C (2018) Quantitative proteomic approaches to characterize the dynamic and comprehensive defence responses of wheat to leaf rust. 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego, CA, USA, June 3-7, 2018. Poster

    2018 - View publication details

  10. González-Peña Fundora D, Subramaniam R, Rampitsch C, Eranthodi A, Thakor N, Foroud NA. 2018. MAPK overexpression in Fusarium graminearum—towards characterization of the Mgv1 signalling pathway. 14th European Fusarium Seminar, Tulln, AUSTRIA, April 8-12, 2018.

    2018 - View publication details

  11. González-Peña Fundora D, Eranthodi A, Goyal RK, Subramaniam R, Rampitsch C, Thakor N, Foroud NA. Over-expression of a Fusarium graminearum mitogen activated protein kinase, Mgv1. National Fusarium Head Blight Forum. Milwaukee WI, UNITED STATES, December 3-5, 2017. Poster Presentation

    2017 - View publication details

  12. Eranthodi A, Subramaniam R, Rampitsch C, González-Peña Fundora D, Shostak K, Goyal RK, Schultz EA, Foroud NA. The role of three small secreted cysteine-rich proteins of Fusarium graminearum on fungal growth and plant infection. National Fusarium Head Blight Forum. Milwaukee WI, UNITED STATES, December 3-5, 2017. Poster Presentation

    2017 - View publication details

  13. Eranthodi A, Subramaniam R, Rampitsch C, González-Peña Fundora D, Goyal RK, Schultz EA, Foroud NA. Characterization of three secreted cysteine-rich proteins from the secretome of Fusarium graminearum. 3rd Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 22, 2017. (Award Winning Presentation) (Oral Presentation)

    2017 - View publication details

  14. Gonzàlez-Peña Fundora D, Eranthodi A, Goyal RK, R. Subramaniam, Rampitsch C, Thakor N, Foroud NA. Generation and characterization of a Fusarium graminearum mutant overexpressing the MAPK, Mgv1. 2017 Joint Meeting of the Canadian Phytopathological Society and the Canadian Society of Agronomy. Winnipeg MB, Canada, June 18-22, 2017. Award Winning Presentation.

    2017 - View publication details

  15. Sivagnanam, K., Komatsu, E., Rampitsch, C., Perreault, H., Gräfenhan, T. (2017). Rapid screening of Alternaria mycotoxins using MALDI-TOF mass spectrometry, 97(1), 357-361. http://dx.doi.org/10.1002/jsfa.7703

    2017 - View publication details

  16. Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Foroud, N.A., and Schultz, E.A. (2016). "Mutant-based screening for pathogen-associated molecular patterns involved in Fusarium head blight disease resistance in wheat.", Prairie University Biology Symposium 2016, The University of Lethbridge, Lethbridge, AB, Canada, February 18-20, 2016. (Poster)

    2016 - View publication details

  17. Gonzalez-Pena Fundora, D., Thakor, N., Subramaniam, R., Rampitsch, C., and Foroud, N.A. (2016). "Towards the identification of the Mgv1 MAPK pathway in Fusarium graminearum.", Prairie University Biology Symposium 2016, The University of Lethbridge, Lethbridge, AB, Canada, February 18-20, 2016. (Poster)

    2016 - View publication details

  18. Eranthodi A, Subramaniam R, Rampitsch C, Ouellet T, Gonzàlez-Peña Fundora D, Vivar M, Zhao W, Goyal RK, Schultz EA, Foroud NA. Fusarium graminearum mutants towards identification of pathogenesis-associated molecular patterns. 2nd Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 30, 2016. Award Winning Presentation

    2016 - View publication details

  19. Foroud, N.A., Eranthodi, A., Goyal, R.K., Gonzàlez-Peña Fundora, D., Zhao, W., Vivar, M., Ouellet. T., Subramaniam, R., Rampitsch, C. 2016. Characterization of a secreted Fusarium graminearum elongation factor – an elicitor of host defence? Proceedings of the 8th Canadian Workshop on Fusarium Head Blight, Ottawa, ON, November 20-22 2016. P 19. Invited Oral Presentation.

    2016 - View publication details

  20. Eranthodi A, Subramaniam R, Rampitsch C, Ouellet T, Vivar M, Gonzàlez-Peña Fundora D, Zhao W, Goyal RK, Schultz EA, Foroud NA. Characterization of three Fusarium graminearum overexpression mutants and their ability to elicit host defence response in cereals. 8th Canadian Workshop of Fusarium Head Blight, Ottawa ON, CANADA, November 20-22, 2016. p.61

    2016 - View publication details

Research facility

Route 100, Unit 100-101
Morden, MB R6M 1Y5
Canada

Affiliations

Editorial Board, Proteomics.

Associate Editor (Microbial Genetics, Genomics and Proteomics), BMC Microbiology.

Language

English

Other languages

Deutsch