Dr Dominic Poulin-Laprade obtained a Ph.D in biology from the University of Sherbrooke in 2015 during which she characterized a genetic regulatory network governing the propagation of mobile genetic elements carrying a multitude of functional genes, including genes for resistance to antibiotics and heavy metals.
In 2016, she began a postdoctoral fellow at the Research and Development Center of Agriculture and Agri-Food Canada in Sherbrooke where she studied the impact of husbandry practices on the intestinal load in antibiotic-resistant bacteria in swine, as well as the modulation of greenhouse gases production by manure having undergone various physicochemical treatments.
Since 2019, Dr. Poulin-Laprade is a research scientist in antimicrobial resistance in pig production for Agriculture and Agri-Food Canada at the Sherbrooke Research and Development Centre in Sherbrooke, Quebec. Her current research program focuses on
the impact of various husbandry practices on; the nature of the genetic determinants conferring antimicrobial resistance, the load of bacterial pathogens resistant to antimicrobials (antibiotics and heavy metals) in the porcine intestinal microbiota and on carcasses intended for human consumption;
modulation of the porcine intestinal microbiota to promote the development of beneficial microorganisms in order to obtain healthier animals, thereby reducing the need to use antibiotics;
- development of approaches to limit the spread of mobile genetic elements carrying antimicrobial resistance genes in the porcine intestinal microbiota, as well as in manures used as land fertilizer.
Current research and/or projects
- Using a «One Health» approach, generating risk assessments for humans, livestock and the environment for resistance genes, their propagating mobile genetic elements, and resistant bacteria of the porcine intestinal microbiota and the farm and abattoir environments.
- Study the impact of husbandry practices and alternatives to antibiotic usage on the composition of intestinal microbial communities, prevalence of antimicrobial resistant bacterial pathogens, and the load in antimicrobial resistance genes and their potential for propagation by conjugation.
- Study of the impact of in-feed copper and zinc supplementation on the load in intestinal bacteria resistant to heavy metals and antibiotics.
- Evaluate the impact of anaerobic digestion of manures on the persistence of bacterial pathogens and antimicrobial resistance genes and their dissemination potential by conjugation.
Professional activities / interests
Researcher of the Swine and Poultry Infectious Diseases Research Center (CRIPA) supported by the Fonds de recherche du Québec – Nature et technologies (FRQNT) strategic cluster n°RS-170946
Member of the local organizing committee for the 2nd International Conference on Holobionts
Education and awards
Philosophiæ Doctor, Ph.D. in biology, University of Sherbrooke, Canada
Doctoral research scholarship B2, Fonds de recherche du Québec-Nature et technologies
Doctoral institutional scholarship, Faculty of Sciences, University of Sherbrooke
Scientific papers (peer review)
- Poulin-Laprade D, Burrus V. (2015) A Cro-like repressor is essential for the induction of conjugative transfer of SXT/R391 elements in response to DNA damage. J Bacteriol, 197(24):3822-3833. Selected for the Spotlight section of the Journal of Bacteriology and featured in an article in mBiosphere.
- Poulin-Laprade D, Matteau D, Jacques PE, Rodrigue S, Burrus V. (2015) Transfer activation of SXT/R391 integrative and conjugative elements: unraveling the SetCD regulon. Nucleic Acids Res, 43(4):2045-56.
- Allard N, Garneau D, Poulin-Laprade D, Burrus V, Brzezinski R, Roy S. (2015) A diaminopimelic acid auxotrophic Escherichia coli donor provides improved counterselection following intergeneric conjugation with actinomycetes. Can J Microbiol, 6(81):565-574.
- Garriss G, Poulin-Laprade D, Burrus V. (2013) DNA damaging agents induce the RecA-independent homologous recombination functions of integrating conjugative elements of the SXT/R391 family. J Bacteriol, 195(9):1991-2003.
- Daccord A, Mursell M, Poulin-Laprade D, Burrus V. (2012) Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family. J Bacteriol, 194(21):5794-802.
- Dubeau MP, Poulin-Laprade D, Ghinet MG, Brzezinski R. (2011) Properties of CsnR, the transcriptional repressor of the chitosanase gene, csnA, of Streptomyces lividans. J Bacteriol, 193(10): 2441-50.
- Ghinet MG, Roy S, Poulin-Laprade D, Lacombe-Harvey M, Morosoli R, Brzezinski R. (2010) Chitosanase from Streptomyces coelicolor A3(2) : biochemical properties and role in protection against antibacterial effect of chitosan. Biochem Cell Biol, 88(6):907-16.
- Poulin-Laprade D, Carraro N, Burrus V. (2015) The extended regulatory networks of SXT/R391 integrative and conjugative elements and IncA/C conjugative plasmids. Regulation of gene expression in enteropathogenic bacteria - Food microbiology. Front Microbiol, 6:837.
- Poulin-Laprade D, Burrus V. (2015) Electrophoretic mobility shift using radioloabeled DNA probes. Methods Mol biol, Springer protocols, 1334:1-15.
Adjunct Professor, Faculty of Sciences, University of Sherbrooke
Member of the Canadian Society of Microbiologists
Member of the American Society for Microbiology